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MMP-9 Antibody - BSA Free

 (10 Publications)
 (4 Reviews)
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Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.
Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.
Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-57940] - Human prostate cancer was detected using HRP/AEC red color stain. Dilution: 2-15 ug/mL.
Western Blot: MMP-9 Antibody [NBP1-57940] - Analysis of MMP-9 in mouse brain tumor lysate (GL261 implanted mouse glioma tissue, 40 ug/lane) using anti-MMP-9 antibody. Image from verified customer review.
Western Blot: MMP-9 Antibody [NBP1-57940] - HeLa cell lysate. Antibody at 1.25 ug/mL.
Western Blot: MMP-9 Antibody [NBP1-57940] - Human Fetal Lung. Antibody at 1.0 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-57940AF350
Alexa Fluor 405
NBP1-57940AF405
Alexa Fluor 488
NBP1-57940AF488
Alexa Fluor 532
NBP1-57940AF532
Alexa Fluor 594
NBP1-57940AF594
Alexa Fluor 647
NBP1-57940AF647
Alexa Fluor 700
NBP1-57940AF700
Alexa Fluor 750
NBP1-57940AF750
Allophycocyanin
NBP1-57940APC
Biotin
NBP1-57940B
CoraFluor 1
NBP1-57940CL1
DyLight 350
NBP1-57940UV
DyLight 405
NBP1-57940V
DyLight 488
NBP1-57940G
DyLight 550
NBP1-57940R
DyLight 594
NBP1-57940DL594
DyLight 650
NBP1-57940C
DyLight 680
NBP1-57940FR
DyLight 755
NBP1-57940IR
FITC
NBP1-57940F
HRP
NBP1-57940H
Janelia Fluor 525
NBP1-57940JF525
Janelia Fluor 549
NBP1-57940JF549
Janelia Fluor 646
NBP1-57940JF646
Janelia Fluor 669
NBP1-57940JF669
PE
NBP1-57940PE
PerCP
NBP1-57940PCP
mFluor Violet 450 SE
NBP1-57940MFV450
mFluor Violet 500 SE
NBP1-57940MFV500
mFluor Violet 610 SE
NBP1-57940MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-57940SS
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MMP-9 Antibody - BSA Free Summary

Immunogen
Synthetic peptide corresponding to the N-terminal of the human MMP-9 protein (between amino acids 150-200) UniProt P14780.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP9
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:250
  • Western Blot 1.25 ug/mL
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.3
using
NBP1-57940 in the following applications:
Publications
Read Publications using
NBP1-57940 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MMP-9 Antibody - BSA Free

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (NBP1-57940)(10)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Sheep.

We have publications tested in 4 applications: IF/IHC, IHC-P, Immunohistochemistry, WB.


Filter By Application
IF/IHC
(2)
IHC-P
(4)
Immunohistochemistry
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-57940 Applications Species
Cengel Kurnaz S, Kuruca N, GUvenC D et al. Topical Administration of Carvacrol Improves Healing in Nasal Septal Perforation: An Experimental Animal Study American journal of rhinology & allergy 2022-03-03 [PMID: 35238647] (IF/IHC) IF/IHC
Kollaras V, Valsami G, Lambropoulou M et al. Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model Acta Cirúrgica Brasileira 2021-11-08 [PMID: 34755764] (Immunohistochemistry) Immunohistochemistry
Vuong TT, Ronning SB, Kolset SO, Pedersen ME. The enzyme profiles in the connective tissue attaching pin bones to the surrounding tissue is specific in farmed salmon (Salmo salar) and cod (Gadus morhua L.) Fish Physiol. Biochem. 2016-07-09 [PMID: 27394140] (IHC-P) IHC-P
Vaithilingam V, Quayum N, Joglekar MV et al. Effect of alginate encapsulation on the cellular transcriptome of human islets. Biomaterials. 2011-09-01 [PMID: 21889795] (WB, Human) WB Human
Cengel Kurnaz S, Aksoy A, Guvenc D. Does Resveratrol Have a Protective Effect on Tympanosclerosis Formation in Experimentally Induced Otitis Media? Int Adv Otol 2 2014-01-01 (IHC-P, Rat) IHC-P Rat
StacY MR, Best CA, Maxfield MW et al. Magnetic Resonance Imaging of Shear Stress and Wall Thickness in Tissue-Engineered Vascular Grafts. Tissue Eng Part C Methods. 2018-07-31 [PMID: 29978768] (IHC-P, Sheep) IHC-P Sheep
De Cunto G, Lamberti A, de Santi MM et al. Middermal Elastolysis: Dermal Fibroblasts Cooperate with Inflammatory Cells to the Elastolytic Disorder Mediators Inflamm. 2017-09-16 [PMID: 29097850] (IF/IHC, Human) IF/IHC Human
Su C Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor kappa B-p65 and cyclooxygenase-2. Mol Med Rep 2016-01-02 [PMID: 26648518] (WB, Human) WB Human
Xu B, Guenther JF, Pociask DA et al. Promotion of Lung Tumor Growth by Interleukin-17. Am. J. Physiol. Lung Cell Mol. Physiol. 2014-07-18 [PMID: 25038189] (IHC-P, Mouse)

Details:
MMP9 antibody used for IHC-P on lungs of K-RasLA1 mice (C57BL/6 background) - autochthonous/lung tumor model (lung delivery of a recombinant adenovirus that expresses IL-17A). Primary dilution 1:200, ON 4C incubation, HRP-DAB detection, IHC images in Fig. 3C. 		IHC-P Mouse
Bellini T, Trentini A, Manfrinato MC, Tamborino C, Volta CA, Di Foggia V, Fainardi E, Dallocchio F, Castellazzi M. Matrix metalloproteinase-9 activity detected in body fluids is the result of two different enzyme forms. J Biochem;151(5):493-499. 2012-05-01 [PMID: 22343748] (WB, Human) WB Human

Reviews for MMP-9 Antibody (NBP1-57940) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-57940:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot MMP-9 NBP1-57940
Enlarge 		4
reviewed by:
Emily Thompson
 WB Human 12/13/2019
View

Summary

ApplicationWestern Blot
Sample Testedconditioned media from human glioma cells,conditioned media from U251 human glioma cell line
SpeciesHuman
LotA-2
Western Blot MMP-9 NBP1-57940
Enlarge 		4
reviewed by:
Emily Thompson
 WB Human 05/02/2019
View

Summary

ApplicationWestern Blot
Sample Testedconcentrated conditioned media,U251 glioma cell line; concentrated conditioned media
SpeciesHuman
LotA-2
Western Blot MMP-9 NBP1-57940
Enlarge 		5
reviewed by:
Verified Customer
 WB Mouse 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedGL261 implanted mouse glioma tissue
SpeciesMouse
LotA
Western Blot MMP-9 NBP1-57940
Enlarge 		4
reviewed by:
Verified Customer
 WB Mouse 07/21/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse glioma tissue
SpeciesMouse
LotQC3458

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP1-57940). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.
  2. I have purchased MMP-9 NBP1-57940 and it is lyophilized. I would like to know how to dilute them for further processing.
    • Our product NBP1-57940 is provided lyophilized. We recommend reconstituting with 0.1ml of dH2O.

Secondary Antibodies

 

Isotype Controls

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Research Areas for MMP-9 Antibody (NBP1-57940)

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
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Western Blot MMP-9 NBP1-57940
Enlarge
 Emily Thompson
12/13/2019
Application: WB
Species: Human
read this review
Western Blot MMP-9 NBP1-57940
Enlarge
 Emily Thompson
05/02/2019
Application: WB
Species: Human
read this review
Western Blot MMP-9 NBP1-57940
Enlarge
 Verified Customer
12/12/2014
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol MMP9
Entrez
Uniprot