MMP-9 Antibody [Unconjugated]

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MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room ...read more
MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue ...read more
MMP-9 was detected in immersion fixed MCF-7 human breast cancer cell line using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue ...read more
Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) ...read more
Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

MMP-9 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Specificity
Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF911 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody [Unconjugated]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (AF911)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 7 applications: ELISA Microarray Development, ICC, IHC, IHC-P, Proximity Ligation Assay (PLA), Western Blot, Zymography.


Filter By Application
ELISA Microarray Development
(1)
ICC
(4)
IHC
(1)
IHC-P
(2)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(4)
Zymography
(1)
All Applications
Filter By Species
Human
(11)
Mouse
(2)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF911 Applications Species
Y Ogura, N Ohbayashi, Y Kanaho, A Kawaguchi, Y Funakoshi Ubiquitin-specific protease TRE17/USP6 promotes tumor cell invasion through the regulation of glycoprotein CD147 intracellular trafficking The Journal of Biological Chemistry, 2022-08-02;0(0):102335. 2022-08-02 [PMID: 35926707] (Zymography, Human) Zymography Human
L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International Journal of Molecular Sciences, 2022-02-10;23(4):. 2022-02-10 [PMID: 35216088] (Western Blot, Human) Western Blot Human
Safiya K, Matias M, Daniel J et al. Splice-switching of the insulin receptor pre-mRNA alleviates tumorigenic hallmarks in rhabdomyosarcoma. NPJ Precis Oncol. 2022-01-11 [PMID: 35017650]
Takako Araki, Jutarat Sangtian, Darin Ruanpeng, Ramachandra Tummala, Brent Clark, Lynn Burmeister et al. Acute elevation of interleukin 6 and matrix metalloproteinase 9 during the onset of pituitary apoplexy in Cushing’s disease Pituitary 2021-12-01 [PMID: 34041660]
M Thanee, H Dokduang, Y Kittirat, J Phetcharab, P Klanrit, A Titapun, N Namwat, N Khuntikeo, A Wangwiwats, H Saya, W Loilome CD44 modulates metabolic pathways and altered ROS-mediated Akt signal promoting cholangiocarcinoma progression PLoS ONE, 2021-03-29;16(3):e0245871. 2021-03-29 [PMID: 33780455] (ICC, Human) ICC Human
Yujing Fan, Kuanyu Wang miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4 Molecular Medicine Reports 2020-05-18 [PMID: 32467998]
E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus J. Cell. Mol. Med., 2018-10-24;23(1):576-585. 2018-10-24 [PMID: 30358100]
Minerva Daya, Watcharin Loilome, Anchalee Techasen, Malinee Thanee, Prakasit Sa-Ngiamwibool, Attapol Titapun et al. Progranulin modulates cholangiocarcinoma cell proliferation, apoptosis, and motility via the PI3K/pAkt pathway OncoTargets and Therapy 2018-02-07 [PMID: 29403285]
ZHI-FEI ZHAO, JIAN-XIONG LI, RUI YE, XUAN WU, LING-LING GAO, BAO-LONG NIU Interleukin-6 as a potential molecular target in esophageal squamous cell carcinoma Oncology Letters 2016-02-01 [PMID: 26893670]
Amir Boufenzer, Jérémie Lemarié, Tabassome Simon, Marc Derive, Youcef Bouazza, Nguyen Tran et al. TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial Infarction Circulation Research 2015-05-22 [PMID: 25840803]
Show All 24 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for MMP-9 Antibody (AF911). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Secondary Antibodies

 

Isotype Controls

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Blogs on MMP-9.

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Bioinformatics

Gene Symbol MMP9