Home » MMP-7 » MMP-7 Antibodies » MMP-7 Antibody (111433) [Unconjugated]

MMP-7 Antibody (111433) [Unconjugated]

 (18 Publications)
 
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Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein ...read more
Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, human small intestine tissue, and CaSki human cervical carcinoma, loaded at 0.5 mg/mL. Specific bands were detected ...read more
Zymography analysis for active MMP2 was performed with 4 μg of total protein of the conditioned medium of U87 (a) and primary IC cells (b) after 48 h exposure to JS-K (1–3 μM) on gelatin-containing PAGE. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clone
111433
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Phycoerythrin
IC9071P
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Carrier Free
IC9071P
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MAB9071-SP
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MAB9071-500
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MMP-7 Antibody (111433) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑7
Leu18-Lys267 (Ala230del)
Accession # NP_002414.1
Specificity
Detects the pro and active forms of human MMP-7 in Western blots. In dot blots, approximately 20% cross-reactivity with recombinant human MMP-8 is observed and no cross-reactivity with recombinant human MMP-1, -2, -3, -9, -10, -12, or -13 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MMP7
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB9071 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-7 Antibody (111433) [Unconjugated]

  • EC 3.4.24
  • EC 3.4.24.23
  • Matrilysin
  • matrin
  • matrix metallopeptidase 7 (matrilysin, uterine)
  • matrix metalloproteinase 7 (matrilysin, uterine)
  • Matrix metalloproteinase-7
  • MMP7
  • MMP-7
  • MPSL1
  • Pump-1 protease
  • PUMP1
  • PUMP-1
  • uterine matrilysin
  • Uterine metalloproteinase

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-7 Antibody (MAB9071)(18)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 6 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(9)
All Applications
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Human
(14)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB9071 Applications Species
M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker Cancers, 2022-07-13;14(14):. 2022-07-13 [PMID: 35884455] (IHC, Human) IHC Human
M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2021-01-11;0(0):. 2021-01-11 [PMID: 33232301] (Western Blot, Human) Western Blot Human
Z Chen, Y Zhu, X Fan, Y Liu, Q Feng Upregulation of long non?coding RNA CCEPR is associated with poor prognosis and contributes to the progression of ovarian cancer through regulating the Wnt/beta?catenin signaling pathway Mol Med Rep, 2020-02-06;21(4):1950-1958. 2020-02-06 [PMID: 32319633] (Western Blot, Human) Western Blot Human
Becky A. S. Bibby, Cecelia S. Miranda, John V. Reynolds, Christopher J. Cawthorne, Stephen G. Maher Silencing microRNA-330-5p increases MMP1 expression and promotes an invasive phenotype in oesophageal adenocarcinoma BMC Cancer 2019-12-01 [PMID: 31391080]
S Caporali, A Amaro, L Levati, E Alvino, PM Lacal, S Mastroeni, F Ruffini, L Bonmassar, GC Antonini C, N Felli, A Carè, U Pfeffer, S D'Atri miR-126-3p down-regulation contributes to dabrafenib acquired resistance in melanoma by up-regulating ADAM9 and VEGF-A J. Exp. Clin. Cancer Res., 2019-06-21;38(1):272. 2019-06-21 [PMID: 31227006] (Western Blot, Human) Western Blot Human
V Alonso, P Escudero, C Fernández-, A Salud, M Méndez, J Gallego, JR Rodriguez, M Martín-Ric, J Fernández-, H Manzano, JC Méndez, M Zanui, E Falcó, M Gil-Raga, F Rojo, M Cuatrecasa, J Feliu, X García-Alb, J Maurel Coexpression of p-IGF-1R and MMP-7 Modulates Panitumumab and Cetuximab Efficacy in RAS Wild-Type Metastatic Colorectal Cancer Patients Neoplasia, 2018-05-26;20(7):678-686. 2018-05-26 [PMID: 29842993] (IHC, Human) IHC Human
C Bolenz, D Knauf, A John, P Erben, A Steidler, SW Schneider, C Günes, C Gorzelanny Decreased Invasion of Urothelial Carcinoma of the Bladder by Inhibition of Matrix-Metalloproteinase 7 Bladder Cancer, 2018-01-20;4(1):67-75. 2018-01-20 [PMID: 29430508] (Western Blot, Human) Western Blot Human
J Guenzle, LJ Wolf, NW Garrelfs, JM Goeldner, N Osterberg, CR Schindler, JE Saavedra, A Weyerbrock ATF3 reduces migration capacity by regulation of matrix metalloproteinases via NF?B and STAT3 inhibition in glioblastoma Cell Death Discov, 2017-02-27;3(0):17006. 2017-02-27 [PMID: 28250971] (Western Blot, Human) Western Blot Human
N Felli, M C Errico, F Pedini, M Petrini, R Puglisi, M Bellenghi et al. AP2 alpha controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression Oncogene 2016-06-01 [PMID: 26434590]
Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces. J. Cell. Sci., 2011-01-11;124(0):369-83. 2011-01-11 [PMID: 21224397] (Flow Cytometry, Human) Flow Cytometry Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol MMP7
Uniprot