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MMP-13 Antibody (87512) [Unconjugated]

 (24 Publications)
 (1 Review)
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MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) overnight at 4 °C. ...read more
Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on ...read more
Enhanced MMP13 gene expression and production due to 3D osteogenic differentiation. (A) Multivariate analysis of the sixty-six tested genes (NanoString® kit) displayed as a volcano plot (created with nSolver® ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, AP, ICC/IF
Clone
87512
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB511-SP
Carrier Free
MAB511-500
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MMP-13 Antibody (87512) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Specificity
Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP13
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoaffinity Purification
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB511 in the following applications:
Publications
Read Publications using
MAB511 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-13 Antibody (87512) [Unconjugated]

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-13 Antibody (MAB511)(24)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Canine.

We have publications tested in 10 applications: ELISA Detection, ELISA Development, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Neutralization, Western Blot.


Filter By Application
ELISA Detection
(1)
ELISA Development
(1)
ICC
(2)
ICC/IF
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Immunohistochemistry
(1)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
Rat
(1)
Bovine
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using MAB511 Applications Species
Ro, DH;Cho, GH;Kim, JY;Min, SK;Yang, HR;Park, HJ;Wang, SY;Kim, YJ;Lee, MC;Bae, HC;Han, HS; Selective targeting of dipeptidyl-peptidase 4 (DPP-4) positive senescent chondrocyte ameliorates osteoarthritis progression Aging cell 2024-03-31 [PMID: 38556837] (Immunohistochemistry, Human, Rat) Immunohistochemistry Human, Rat
Koulicoff, LA;Heilman, T;Vitanza, L;Welter, A;Jeneske, H;O'Quinn, TG;Hansen, S;Huff-Lonergan, E;Schulte, MD;Chao, MD; Matrix metalloproteinase- 9 may contribute to collagen structure modification during postmortem aging of beef Meat science 2023-08-25 [PMID: 37643525] (Western Blot, Bovine) Western Blot Bovine
Li P, Lv S, Jiang W et al. Exosomes derived from umbilical cord mesenchymal stem cells protect cartilage and regulate the polarization of macrophages in osteoarthritis Annals of Translational Medicine 2022-09-01 [PMID: 36267713]
E Pellegrini, G Desando, M Petretta, A Cellamare, C Cristalli, M Pasello, MC Manara, B Grigolo, K Scotlandi A 3D Collagen-Based Bioprinted Model to Study Osteosarcoma Invasiveness and Drug Response Polymers, 2022-09-28;14(19):. 2022-09-28 [PMID: 36236019] (ICC, Human) ICC Human
M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker Cancers, 2022-07-13;14(14):. 2022-07-13 [PMID: 35884455] (IHC, Human) IHC Human
Oliveros Anerillas L, Kingham PJ, Lammi MJ et al. Three-Dimensional Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells Promotes Matrix Metallopeptidase 13 (MMP13) Expression in Type I Collagen Hydrogels International journal of molecular sciences 12/18/2021 [PMID: 34948393]
RT Ormsby, AR Zelmer, D Yang, NJ Gunn, Y Starczak, SP Kidd, R Nelson, LB Solomon, GJ Atkins Evidence for osteocyte-mediated bone-matrix degradation associated with periprosthetic joint infection (PJI) European cells & materials, 2021-10-08;41(0):264-280. 2021-10-08 [PMID: 34622431] (IHC, Human) IHC Human
Sunghyun Park, Yoshie Arai, Alvin Bello, Hansoo Park, Dohyun Kim, Kyung-Soon Park et al. SPRY4 acts as an indicator of osteoarthritis severity and regulates chondrocyte hypertrophy and ECM protease expression npj Regenerative Medicine 9/17/2021 [PMID: 34535669]
Jinsung Park, Hyosun Park, Young Lim Lee, Subin Weon, Yong-Gil Kim, Jae-Hyuk Yang et al. Blocking TNF alpha attenuates progressive cartilage matrix degradation in inflammatory arthritis Experimental and Therapeutic Medicine 2021-05-27 [PMID: 34093764]
Amanda Villalvilla, Ane Larrañaga-Vera, Ana Lamuedra, Sandra Pérez-Baos, Alberto G. López-Reyes, Gabriel Herrero-Beaumont et al. Modulation of the Inflammatory Process by Hypercholesterolemia in Osteoarthritis Frontiers in Medicine 2020-09-25 [PMID: 33102504]
Show All 24 Publications.

Review for MMP-13 Antibody (MAB511) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB511:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Mozhdeh Sojoodi
 ICC Mouse 02/07/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lot1806

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
1:200 Dilution
Overnight incubation at RT

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Immunocytochemistry MMP-13 MAB511
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 Mozhdeh Sojoodi
02/07/2022
Application: ICC
Species: Mouse
read this review

Bioinformatics

Gene Symbol MMP13
Uniprot