![Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Western-Blot-NBP2-72687-img0002.jpg "Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | OTI9E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FERMT2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 77.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FERMT2 |
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0010.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72687-img0003.jpg "Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.")
![Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Western-Blot-NBP2-72687-img0001.jpg "Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.")
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0004.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0005.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0006.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0007.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0008.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72687-img0009.jpg "Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Azide-and-BSA-Free-Immunoprecipitation-NBP2-72687-img0011.jpg "Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
![Western Blot SOS2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOS2_AF6260_Western_Blot_9542.jpg)
