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MFRP Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3445
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Catalog# & Formulation Size Price
Carrier Free
AF3445-SP
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MFRP Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MFRP
Gln91-Pro584
Accession # Q8K480
Specificity
Detects mouse MFRP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) MFRP is observed, 15% cross-reactivity with recombinant mouse (rm) Frizzled-4 is observed, 10% cross-reactivity with rmFrizzled-1, rhFrizzled-5, rmFrizzled-8, and rmFrizzled-10 is observed, and 5% cross-reactivity with rmFrizzled-3, rmFrizzled-6, and rmFrizzled-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MFRP
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3445 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFRP Antibody

  • MCOP5
  • membrane frizzled-related protein
  • Membrane-type frizzled-related protein
  • MFRP
  • MGC32938
  • NNO2
  • rd6

Background

MFRP is a type II transmembrane protein that is related to a small family of soluble frizzled-related proteins. It is the product of a dicistronic mRNA transcript that codes for two proteins; MFRP and C1qTNF5. Mouse MFRP is 584 amino acids (aa) in length. Its extracellular domain (ECD) of 494 aa contains two CUB and two LDLR class A segments, plus one frizzled domain. At least two splice variants are known in mouse. In isoform 2, amino acid residues 425‑431 of isoform 1 is substituted with Lys. Mouse MFRP ECD shares 72% aa identity with human MFRP ECD. In mouse retinal degeneration 6, MFRP is mutated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MFRP Antibody (AF3445)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Electron Microscopy, IHC, IHC-Fr, Western Blot.


Filter By Application
Electron Microscopy
(1)
IHC
(1)
IHC-Fr
(1)
Western Blot
(1)
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Mouse
(2)
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Product General Protocols

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Bioinformatics

Gene Symbol MFRP
Uniprot