![Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Western blotting using Mesothelin antibody (MB-G10). Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng)Primary antibody: anti-Mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MB-G10-Western-Blot-NB110-85538-img0008.jpg "Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Western blotting using Mesothelin antibody (MB-G10). Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng)Primary antibody: anti-Mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, PAGE |
| Clone | MB-G10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This product was purified from tissue culture supernatant fluid by Protein A chromatography Store antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This Mesothelin Antibody (MB-G10) was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human Mesothelin full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. (Uniprot: Q13421) |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MSLN |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For Anti-mesothelin immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Mesothelin Antibody (NB110-85538)Find related products by research area.
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![Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538] - Immunohistochemistry using Mesothelin antibody (MB-G10) to react with two epitopes on Mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-Mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to Equineradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MB-G10-Immunohistochemistry-NB110-85538-img0010.jpg "Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538] - Immunohistochemistry using Mesothelin antibody (MB-G10) to react with two epitopes on Mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-Mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to Equineradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.")
![SDS-Page: Mesothelin Antibody (MB-G10) [NB110-85538] - SDS-PAGE of Mesothelin antibody (MB-G10). Lane 1: Non-Reduced Mesothelin antibody (MB-G10). Lane M: 3 uL OPAL Pre-stained Marker. Lane 2: Reduced Mesothelin antibody (MB-G10). Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Mesothelin-Antibody-MB-G10-SDS-Page-NB110-85538-img0009.jpg "SDS-Page: Mesothelin Antibody (MB-G10) [NB110-85538] - SDS-PAGE of Mesothelin antibody (MB-G10). Lane 1: Non-Reduced Mesothelin antibody (MB-G10). Lane M: 3 uL OPAL Pre-stained Marker. Lane 2: Reduced Mesothelin antibody (MB-G10). Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa.")
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