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Mesothelin Antibody (MB-G10) [mFluor Violet 610 SE]

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Mesothelin Antibody (MB-G10) [mFluor Violet 610 SE] [NB110-85538MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, PAGE
Clone
MB-G10
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 610 SE

Order Details

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Mesothelin Antibody (MB-G10) [mFluor Violet 610 SE] Summary

Immunogen
This Mesothelin Antibody (MB-G10) was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human Mesothelin full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. (Uniprot: Q13421)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MSLN
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • SDS-Page
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Cross reactivity with homologues from other sources has not been tested

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mesothelin Antibody (MB-G10) [mFluor Violet 610 SE]

  • CAK1 antigen
  • CAK1
  • megakaryocyte potentiating factor
  • Mesothelin
  • MPF
  • MPFSMRP
  • MSLN
  • Pre-pro-megakaryocyte-potentiating factor
  • SMR
  • soluble MPF mesothelin related protein

Background

MSLN, also known as Mesothelin, has 4 isoforms, a 630 amino acid isoform that is 69 kDa, a 622 amino acid isoform that is 68 kDa, a 656 amino acid isoform that is 71 kDa, and a 621 amino acid isoform that is 68 kDa, expressed in lung, heart, placenta kidney, and overexpressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas. Membrane-anchored forms may play a role in cellular adhesion and megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Current research is being performed on several diseases and disorders including squamous cell carcinoma, ovarian cancer, carcinoma, malignant biphasic mesothelioma, bile duct adenoma, malignant mesothelioma, malignant pleural mesothelioma, gastrointestinal stromal tumor, renal clear cell carcinoma, epithelioid sarcoma, benign mesothelioma, pancreatic ductal adenocarcinoma, asbestosis, pancreatic ductal carcinoma, thymic carcinoma, acute myeloid leukemia, epithelial ovarian cancer, myeloid leukemia, thoracic cancer, and pancreatitis. This protein has also shown an interaction with MUC16 and RSG1 in cell adhesion and pancreas development processes and pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Mesothelin Antibody (NB110-85538MFV610) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSLN