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MDC1 Antibody [Janelia Fluor® 585]

Name: MDC1 Antibody [Janelia Fluor&#174; 585]
Brand: Novus Biologicals
SKU: NB100-397JF585
Price: 549 USD

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Product Details

Summary

Reactivity	Hu, PmSpecies Glossary
Applications	WB, ICC/IF, IHC, IP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Janelia Fluor 585

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-397AF350

Alexa Fluor 405 NB100-397AF405

Alexa Fluor 488 NB100-397AF488

Alexa Fluor 532 NB100-397AF532

Alexa Fluor 594 NB100-397AF594

Alexa Fluor 647 NB100-397AF647

Alexa Fluor 700 NB100-397AF700

Alexa Fluor 750 NB100-397AF750

Allophycocyanin NB100-397APC

Biotin NB100-397B

CoraFluor 1 NB100-397CL1

DyLight 350 NB100-397UV

DyLight 405 NB100-397V

DyLight 488 NB100-397G

DyLight 550 NB100-397R

DyLight 594 NB100-397DL594

DyLight 650 NB100-397C

DyLight 680 NB100-397FR

DyLight 755 NB100-397IR

FITC NB100-397F

HRP NB100-397H

Janelia Fluor 525 NB100-397JF525

Janelia Fluor 549 NB100-397JF549

Janelia Fluor 635 NB100-397JF635

Janelia Fluor 646 NB100-397JF646

Janelia Fluor 669 NB100-397JF669

PE NB100-397PE

PerCP NB100-397PCP

Unconjugated NB100-397

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-397AF350
Alexa Fluor 405	NB100-397AF405
Alexa Fluor 488	NB100-397AF488
Alexa Fluor 532	NB100-397AF532
Alexa Fluor 594	NB100-397AF594
Alexa Fluor 647	NB100-397AF647
Alexa Fluor 700	NB100-397AF700
Alexa Fluor 750	NB100-397AF750
Allophycocyanin	NB100-397APC
Biotin	NB100-397B
CoraFluor 1	NB100-397CL1
DyLight 350	NB100-397UV
DyLight 405	NB100-397V
DyLight 488	NB100-397G
DyLight 550	NB100-397R
DyLight 594	NB100-397DL594
DyLight 650	NB100-397C
DyLight 680	NB100-397FR
DyLight 755	NB100-397IR
FITC	NB100-397F
HRP	NB100-397H
Janelia Fluor 525	NB100-397JF525
Janelia Fluor 549	NB100-397JF549
Janelia Fluor 635	NB100-397JF635
Janelia Fluor 646	NB100-397JF646
Janelia Fluor 669	NB100-397JF669
PE	NB100-397PE
PerCP	NB100-397PCP
Unconjugated	NB100-397

MDC1 Antibody [Janelia Fluor® 585] Summary

Immunogen	The immunogen this antibody was made to, maps to a region between residues 1375 and 1425 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656).
Predicted Species	Primate (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	MDC1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Paraffin Immunoprecipitation Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee.

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MDC1 Antibody [Janelia Fluor® 585]

DKFZp781A0122
Em:AB023051.5
KIAA0170MGC166888
MDC1
mediator of DNA damage checkpoint 1
mediator of DNA damage checkpoint protein 1
mediator of DNA-damage checkpoint 1
NFBD1
NFBD1homologue to Drosophila photoreceptor protein calphotin
Nuclear factor with BRCT domains 1

Background

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MDC1 Antibody
[Janelia Fluor®

NB100-397JF585

Species: Hu, Pm

Applications: WB, ICC/IF, IHC, IP

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Product General Protocols

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Video Protocols

WB Video Protocol

ICC/IF Video Protocol

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Secondary Antibodies

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Blogs on MDC1.

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Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis. This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry. While normal rates of DNA ... Read full blog post.

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Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.

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Bioinformatics

Gene Symbol

MDC1

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

MDC1 Antibody [Janelia Fluor® 585]

Species Glossary

Images

Product Details

Order Details

MDC1 Antibody [Janelia Fluor® 585] Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Notes

Alternate Names for MDC1 Antibody [Janelia Fluor® 585]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for MDC1 Antibody (NB100-397JF585) (0)

Reviews for MDC1 Antibody (NB100-397JF585) (0)

Product General Protocols

Video Protocols

FAQs for MDC1 Antibody (NB100-397JF585) (0)

Secondary Antibodies

Isotype Controls

Additional MDC1 Products

Research Areas for MDC1 Antibody (NB100-397JF585)

Blogs on MDC1.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics