![Western Blot: MBP Antibody (7G7) [NBP1-05203] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05203, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.](http://images.novusbio.com/fullsize/MBP-Antibody-7G7-Western-Blot-NBP1-05203-img0002.jpg "Western Blot: MBP Antibody (7G7) [NBP1-05203] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05203, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.")
| Reactivity | Hu, Mu, Rt, Po, Bv, EqSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 7G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Purified myelin basic protein isolated from bovine brain, epitope is in peptide TPPPSQGKG, amino acids 125-133 of the human 21.5kDa sequence. |
| Marker | Oligodendrocyte Marker, Myelin Marker |
| Specificity | The MBP Antibody (7G7) antibody binds all four of the CNS MBP isoforms, so that the epitope for the antibody is located in the core shared by all four gene products. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MBP |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Immunoprecipitation reported in scientific literature (PMID:34880248).This Myelin Basic Protein (7G7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In WB bands can be seen at ~18 and 21.5 kDa. Use in IHC-Fr was reported in scientific literature (PMID: 26482371).. |
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| Theoretical MW | 19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MBP Antibody (NBP1-05203)Find related products by research area.
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![Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203] - Analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), NBP1-05203, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H, dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.](http://images.novusbio.com/fullsize/MBP-Antibody-7G7-Immunocytochemistry-Immunofluorescence-NBP1-05203-img0001.jpg "Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203] - Analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), NBP1-05203, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H, dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.")
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