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MARCKS Antibody (2C2)

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Western Blot: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of MARCKS expression in Raw 264.7. (The predicted M.W. of MARCKS is 29 to 39 KDa, but it seems the actual M.W. in vivois between 68 to 90 KDa in different species.)
Western Blot: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of MARCKS expression in Raw 264.7. (The predicted M.W. of MARCKS is 29 to 39 KDa, but it seems the actual M.W. in vivois between 68 to 90 KDa in different ...read more
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of monoclonal antibody to MARCKS on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of monoclonal antibody to MARCKS on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml
ELISA: MARCKS Antibody (2C2) [H00004082-M06] - Detection limit for recombinant GST tagged MARCKS is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MARCKS Antibody (2C2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MARCKS (NP_002347, 2 a.a. ~ 65 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAP
Specificity
MARCKS - myristoylated alanine-rich protein kinase C substrate
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MARCKS
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MARCKS Antibody (2C2)

  • 80K-L protein
  • 80K-L
  • FLJ14368
  • MACS
  • MACSmyristoylated alanine-rich C-kinase substrate
  • MARCKS
  • MRACKS
  • myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
  • myristoylated alanine-rich protein kinase C substrate
  • phosphomyristin
  • PKCSLFLJ90045
  • PRKCSL
  • Protein kinase C substrate, 80 kDa protein, light chain

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MARCKS Antibody (H00004082-M06). (Showing 1 - 1 of 1 FAQ).

  1. I need technical information about the MARCKS antibody 2C2 (H00004082-M06). I would like to have more information about the immunogen used. Are the aa corresponding to the effector domain?
    • The immunogen for this antibody is 'MARCKS (NP_002347, 2 a.a. - 66 a.a) partial recombinant protein with GST tag. GAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKV NGDASPAAAESGAKEELQANGSAP'. The MARCKS effector domain corresponds to amino acids 151-175, KKKKKRFSFKKSFKLSGFSFKKNKK-NH (see JBC and PMC) for more information). Therefore, the answer is no.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MARCKS
Entrez
OMIM
Uniprot