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Mannose Phosphate Isomerase Antibody (OTI4G7) - Azide and BSA Free

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Western Blot: Mannose Phosphate Isomerase Antibody (OTI4G7) - Azide and BSA Free [NBP2-72584] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate.
Flow Cytometry: Mannose Phosphate Isomerase Antibody (OTI4G7) - Azide and BSA Free [NBP2-72584] - Analysis of Hela cells, using MPI antibody (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI4G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Mannose Phosphate Isomerase Antibody (OTI4G7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MPI(NP_002426) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MPI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
Theoretical MW
46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Mannose Phosphate Isomerase Antibody (OTI4G7) - Azide and BSA Free

  • CDG1B
  • EC 5.3.1
  • EC 5.3.1.8
  • FLJ39201
  • mannose phosphate isomerase
  • mannose-6- phosphate isomerase
  • mannose-6-phosphate isomerase
  • Phosphohexomutase
  • phosphomannose isomerase 1
  • Phosphomannose isomerase
  • PMI
  • PMI1phosphohexomutase

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MPI