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MAML1 Antibody

Images

 
Western Blot: MAML1 Antibody [NB100-1306] - (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MAML1 Antibody [NB100-1306] - 3.8ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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MAML1 Antibody Summary

Immunogen
Peptide with sequence VLPTCPMAEFALPR corresponding to N-Terminus according to NP_055572.1.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAML1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human skeletal muscle lysates (calculated MW of 108 kDa band according to NP_055572.1). IHC-P: Human placenta shows nuclear staining in trophoblasts.
Control
Jurkat Whole Cell Lysate
Human Ovary Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1306.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAML1 Antibody

  • FLJ53540
  • KIAA0200Mam1
  • Mam-1mastermind homolog
  • mastermind (drosophila)-like 1
  • mastermind-like 1 (Drosophila)
  • mastermind-like protein 1

Background

MAML1 is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAML1 Antibody (NB100-1306)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MAML1 Antibody (NB100-1306) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MAML1 Products

Array NB100-1306

Research Areas for MAML1 Antibody (NB100-1306)

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Blogs on MAML1

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Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol MAML1
Entrez
Uniprot