MALT1 Antibody (MT1/410) Summary
Description |
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33054)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen |
Human MALT1 recombinant fragment (aa701-808) (Uniprot: Q9UDY8) |
Localization |
Cytoplasmic |
Marker |
MALT-Lymphoma Marker |
Isotype |
IgG1 Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
MALT1 |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1-2 ug/ml
- Simple Western 10 ug/ml
- Western Blot 1-2 ug/ml
|
Application Notes |
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. |
Theoretical MW |
93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C. |
Buffer |
10 mM PBS with 0.05% BSA |
Preservative |
0.05% Sodium Azide |
Concentration |
0.2 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for MALT1 Antibody (MT1/410)
Background
MALT1/Paracaspase (MALT1) was independently identified as a member of the human paracaspase family and an interacting partner of B-cell lymphoma (Uren et al., 2000). MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain. It binds to Bcl-10 through its Ig-like domains and cooperates with Bcl-10 to activate NF-kappaB. MALT1 is thought to play an important role in NF-kappaB signaling by enchancing NF-kappaB activation through interaction with Bcl-10 (Ho et al., 2005). Interaction between MALT1 and Bcl-10 mediates IKK activation in vitro through facilitatiing the ubiquitination of NEMO by the ubiquitin-conjugating enzyme UBC13.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for MALT1 Antibody (NBP2-29430). (Showing 1 - 2 of 2 FAQ).
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What's difference between NBP2-32872-0.1mg and NBP2-29430?
- NBP2-32872-0.1mg and NBP2-29430 were raised against the same immunogen but are different clones, so they recognize a different epitope. NBP2-32872 is protein A purified, and NBP2-29430 is protein G purified.
-
NBP2-29430 has been tested for flow. What type of stainings (surface or intracellular) was tested to confirm this Ab work for flow in your company?
- MALT1 is a cytoplasmic protein, so you will have to do intracellular staining.
Secondary Antibodies
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