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MALT1 Antibody (MT1/410)

Images

 
Western Blot: MALT1 Antibody (MT1/410) [NBP2-29430] - Western Blot Analysis of Daudi cell Lysate using MALT1 Monoclonal Antibody (MT1/410)
Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430] - Formalin-fixed, paraffin-embedded tonsil stained with MALT1 Ab (MT1/410)
Immunohistochemistry-Paraffin: MALT1 Antibody (MT1/410) [NBP2-29430] - Formalin-fixed, paraffin-embedded human tonsil stained with MALT1 MT1/410 antibody (1:200), peroxidase-conjugate and DAB chromogen.
Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430] - Simple Western lane view shows a specific band for MALT1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 ...read more
Simple Western: MALT1 Antibody (MT1/410) [NBP2-29430] - Electropherogram image of the corresponding Simple Western lane. MALT1 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
MT1/410
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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MALT1 Antibody (MT1/410) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33054)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Human MALT1 recombinant fragment (aa701-808) (Uniprot: Q9UDY8)
Localization
Cytoplasmic
Marker
MALT-Lymphoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MALT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MALT1 Antibody (MT1/410)

  • caspase-like protein
  • DKFZp434L132
  • EC 3.4.22.-
  • MALT associated translocation
  • MALT lymphoma-associated translocation
  • MALT1
  • MLT1
  • MLTMALT-lymphoma associated translocation
  • mucosa associated lymphoid tissue lymphoma translocation gene 1
  • mucosa-associated lymphoid tissue lymphoma translocation protein 1
  • Paracaspase

Background

MALT1/Paracaspase (MALT1) was independently identified as a member of the human paracaspase family and an interacting partner of B-cell lymphoma (Uren et al., 2000). MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain. It binds to Bcl-10 through its Ig-like domains and cooperates with Bcl-10 to activate NF-kappaB. MALT1 is thought to play an important role in NF-kappaB signaling by enchancing NF-kappaB activation through interaction with Bcl-10 (Ho et al., 2005). Interaction between MALT1 and Bcl-10 mediates IKK activation in vitro through facilitatiing the ubiquitination of NEMO by the ubiquitin-conjugating enzyme UBC13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MALT1 Antibody (NBP2-29430). (Showing 1 - 2 of 2 FAQ).

  1. What's difference between NBP2-32872-0.1mg and NBP2-29430?
    • NBP2-32872-0.1mg and NBP2-29430 were raised against the same immunogen but are different clones, so they recognize a different epitope. NBP2-32872 is protein A purified, and NBP2-29430 is protein G purified.
  2. NBP2-29430 has been tested for flow. What type of stainings (surface or intracellular) was tested to confirm this Ab work for flow in your company?
    • MALT1 is a cytoplasmic protein, so you will have to do intracellular staining.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MALT1
Uniprot