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MAGEB1 Antibody (OTI4E12)

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Western Blot: MAGEB1 Antibody (4E12) [NBP2-45801] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; ...read more
Immunohistochemistry: MAGEB1 Antibody (4E12) [NBP2-45801] - Analysis of Carcinoma of Human bladder tissue.
Western Blot: MAGEB1 Antibody (4E12) [NBP2-45801] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEB1.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, IHC
Clone
OTI4E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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MAGEB1 Antibody (OTI4E12) Summary

Immunogen
Full length human recombinant protein of human MAGEB1 (NP_796379) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAGEB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAGEB1 Antibody (OTI4E12)

  • Cancer/testis antigen 3.1
  • CT3.1cancer/testis antigen family 3, member 1
  • DAM10MAGE-B1 antigen
  • DSS/AHC critical interval MAGE superfamily 10
  • DSS-AHC critical interval MAGE superfamily 10
  • MAGEL1MAGE-XP antigen
  • MAGEXP
  • MAGE-Xp
  • melanoma antigen family B, 1
  • melanoma-associated antigen B1
  • MGC9322

Background

MAGEB1 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene, however, the full length nature of some variants has not been defined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAGEB1