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LRRK2 Antibody [Alexa Fluor® 647]

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Flow Cytometry: LRRK2 Antibody [Alexa Fluor® 647] [NB300-268AF647] - An intracellular stain was performed on U2OS cells with LRRK Antibody NB300-268AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: LRRK2 Antibody [Alexa Fluor (R) 647] [NB300-268AF647] - analysis of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody conjugated with AF647. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ce, I, PlSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

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LRRK2 Antibody [Alexa Fluor® 647] Summary

Immunogen
A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]
Localization
Mitochondrial outer membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NB300-268AF647 in the following applications:

Publications
Read Publication using
NB300-268AF647 in the following applications:

Reactivity Notes

Human, Moth reactivity reported in scientific literature (PMID: 19824698). Plant reactivity reported in scientific literature (PMID: 27273569).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for LRRK2 Antibody [Alexa Fluor® 647]

  • augmented in rheumatoid arthritis 17
  • AURA17
  • Dardarin
  • DKFZp434H2111
  • EC 2.7.11.1
  • FLJ45829
  • leucine-rich repeat kinase 2
  • leucine-rich repeat serine/threonine-protein kinase 2
  • LRRK2
  • PARK8
  • PARK8DARDARIN
  • Parkinson disease (autosomal dominant) 8
  • RIPK7
  • ROCO2

Background

LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

LRRK2 antibodies are useful tools for neurodegeneration research and certian kinase signaling pathway studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRRK2 Antibody (NB300-268AF647)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: FLOW.


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Review for LRRK2 Antibody (NB300-268AF647) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-268AF647:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry LRRK2 NB300-268AF647
Enlarge
5
reviewed by:
Corinna Bliederhaeuser
Flow Human 01/14/2015
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Summary

ApplicationFlow Cytometry
Sample Testednormal human peripheral blood cells (anticoagulated Lithium-Heparin)
SpeciesHuman
LotE-2-AF647

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LRRK2 Antibody (NB300-268AF647) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LRRK2 Products

Research Areas for LRRK2 Antibody (NB300-268AF647)

Find related products by research area.

Blogs on LRRK2.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Parkinson's Disease Infographic
Parkinson's disease affects the nervous system which controls movement. Damage to the levels of dopamine in the brain impairs the ability to relay messages to parts of the body which control movement. While the exact cause of the disease is unknown, r...  Read full blog post.

Novus Antibodies Highlighted in Parkinson's Disease Research
Identified almost two centuries ago, Parkinson's disease is a neurodegenerative disorder that afflicts an estimated 4-6 million worldwide (www.parkinsons.org). The prevalence of Parkinson's disease is expected to grow considerably as the average age o...  Read full blog post.

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Recent Reviews

5
5
1
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1
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Corinna Bliederhaeuser
01/14/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol LRRK2