![Western Blot: Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis using LRP1 mAb against human LRP1 (AA: 20-155) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Low-Density-LRP-Antibody-1F6C6-Western-Blot-NBP2-52507-img0003.jpg "Western Blot: Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis using LRP1 mAb against human LRP1 (AA: 20-155) recombinant protein. (Expected MW is 40.8 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | 1F6C6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human LRP-1 (AA: 20-155) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LRP1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 504 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LRP1 |
![Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (1F6C6) [NBP2-52507] - Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis of HeLa cells using LRP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Low-Density-LRP-Antibody-1F6C6-Immunofluorescence-NBP2-52507-img0002.jpg "Immunocytochemistry/Immunofluorescence: LRP-1 Antibody (1F6C6) [NBP2-52507] - Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis of HeLa cells using LRP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Western Blot: Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis using LRP1 mAb against HEK293 (1) and LRP1 (AA: 20-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Low-Density-LRP-Antibody-1F6C6-Western-Blot-NBP2-52507-img0004.jpg "Western Blot: Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis using LRP1 mAb against HEK293 (1) and LRP1 (AA: 20-155)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis of Raji cells using LRP1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Low-Density-LRP-Antibody-1F6C6-ELISA-NBP2-52507-img0001.jpg "ELISA: Low Density LRP Antibody (1F6C6) [NBP2-52507] - Analysis of Raji cells using LRP1 mouse mAb (green) and negative control (red).")
![ELISA: LRP-1 Antibody (1F6C6) [NBP2-52507] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/LRP-1-Antibody-1F6C6-ELISA-NBP2-52507-img0005.jpg "ELISA: LRP-1 Antibody (1F6C6) [NBP2-52507] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")