LRH-1/NR5A2 Antibody (H2325) [Unconjugated] Summary
Immunogen Recombinant human LRH-1/NR5A2
Specificity This antibody specifically recognizes human LRH-1 alpha and LRH-1 beta and
cross-reacts with mouse and rat LRH-1. Not yet tested in other species.
Source N/A
Isotype IgG3
Clonality Monoclonal
Host Mouse
Gene NR5A2
Purity Statement Protein A or G purified from hybridoma culture supernatant
Innovator's Reward Test in a species/application not listed above to receive a full credit towards a future purchase.
Applications/Dilutions
Dilutions
Direct ELISA Immunoprecipitation Western Blot
Publications
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Packaging, Storage & Formulations
Storage This antibody is stable for greater than six months when held at -20 °C in a
manual defrost freezer or at -70 °C. Upon thawing, the antibody can be
stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid
repeated freeze-thaw cycles.
Buffer A liquid formulation in physiologic saline with 0.1% NaN3 .
Preservative 0.1% Sodium Azide
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for LRH-1/NR5A2 Antibody (H2325) [Unconjugated]
Background
Liver Receptor Homologous Protein (LRH-1, FTF, CPF, hB1F, FTZ-F1b; NR5A2) is a member of the Orphan Nuclear Receptor family. LRH-1 is a mammalian homolog of the Drosophila Fushi Tarazu F1 Gene product. LRH-1 is expressed in the liver and intestine. LRH-1 is involved in the regulation of genes which participate in steroid, bile acid and cholesterol homeostasis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for LRH-1/NR5A2 Antibody (PP-H2325-00)(15)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 5 applications: ChIP, IHC, Immunocytochemistry, Immunoprecipitation, Western Blot.
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Showing Publications 1 -
10 of 15.
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Publications using PP-H2325-00
Applications
Species
Nguyen, NMP;Chang, EM;Chauvin, M;Sicher, N;Kashiwagi, A;Nagykery, N;Chow, C;May, P;Mermin-Bunnel, A;Cleverdon, J;Duong, T;Meinsohn, MC;Gao, D;Donahoe, PK;Pepin, D; AMH protects the ovary from doxorubicin by regulating cell fate and the response to DNA damage bioRxiv : the preprint server for biology 2024-05-23 [PMID: 38826466] (Immunocytochemistry, Mouse) Immunocytochemistry
Mouse
Birgersson, M;Indukuri, R;Lindquist, L;Stepanauskaite, L;Luo, Q;Deng, Q;Archer, A;Williams, C; Ovarian ER? cistrome and transcriptome reveal chromatin interaction with LRH-1 BMC biology 2023-11-29 [PMID: 38031019] (ChIP, Mouse) ChIP
Mouse
J Son, W Du, M Esposito, K Shariati, H Ding, Y Kang, D Accili Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of beta-cell failure Nature Communications, 2023-02-02;14(1):558. 2023-02-02 [PMID: 36732513] (IHC, Mouse) IHC
Mouse
Yu Sun, Hadrien Demagny, Adrien Faure, Francesca Pontanari, Antoine Jalil, Nadia Bresciani et al. Asparagine protects pericentral hepatocytes during acute liver injury Journal of Clinical Investigation 2023-04-03 [PMID: 36719750]
KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler Reprogramming competence of OCT factors is determined by transactivation domains Science Advances, 2020-09-02;6(36):. 2020-09-02 [PMID: 32917606] (Western Blot, Mouse) Western Blot
Mouse
Corey D. Seacrist, Georg Kuenze, Reece M. Hoffmann, Brandon E. Moeller, John E. Burke, Jens Meiler et al. Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and Function Structure 2020-07-01 [PMID: 32433991]
A Milona, V Massafra, H Vos, J Naik, N Artigas, HAB Paterson, ITGW Bijsmans, ECL Willemsen, JM Ramos Pitt, I Miguel-Ali, P Bosma, BMT Burgering, C Williamson, S Vernia, WS Dhillo, SWC van Mil, BM Owen Steroidogenic control of liver metabolism through a nuclear receptor-network Mol Metab, 2019-09-30;30(0):221-229. 2019-09-30 [PMID: 31767173] (ChIP, Mouse) ChIP
Mouse
JR Bayrer, H Wang, R Nattiv, M Suzawa, HS Escusa, RJ Fletterick, OD Klein, DD Moore, HA Ingraham LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival Nat Commun, 2018-10-10;9(1):4055. 2018-10-10 [PMID: 30305617] (Western Blot, Human) Western Blot
Human
A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis J. Biol. Chem., 2018-04-17;0(0):. 2018-04-17 [PMID: 29666185] (Immunoprecipitation, Human) Immunoprecipitation
Human
DA Miranda, WC Krause, A Cazenave-G, M Suzawa, H Escusa, JC Foo, DS Shihadih, A Stahl, M Fitch, E Nyangau, M Hellerstei, MR Wenk, DL Silver, HA Ingraham LRH-1 regulates hepatic lipid homeostasis and maintains arachidonoyl phospholipid pools critical for phospholipid diversity JCI Insight, 2018-03-08;3(5):. 2018-03-08 [PMID: 29515023] (Western Blot, Mouse) Western Blot
Mouse
Martin Wagner, Sungwoo Choi, Katrin Panzitt, Jennifer L. Mamrosh, Jae Man Lee, Alex Zaufel et al. Liver receptor homolog‐1 is a critical determinant of methyl‐pool metabolism Hepatology 2016-01-01 [PMID: 26267291]
Bayrer J, Mukkamala S, Sablin E, Webb P, Fletterick R Silencing LRH-1 in colon cancer cell lines impairs proliferation and alters gene expression programs. Proc Natl Acad Sci U S A, 2015-02-09;112(8):2467-72. 2015-02-09 [PMID: 25675535] (Western Blot, Human) Western Blot
Human
Hansook Kim Chong, Jacob Biesinger, Young-Kyo Seo, Xiaohui Xie, Timothy F Osborne Genome-wide analysis of hepatic LRH-1 reveals a promoter binding preference and suggests a role in regulating genes of lipid metabolism in concert with FXR BMC Genomics 2012-12-01 [PMID: 22296850]
Holmstrom SR, Deering T, Swift GH LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function. Genes Dev., 2011-08-15;25(16):1674-9. 2011-08-15 [PMID: 21852532] (ChIP, Mouse) ChIP
Mouse
Guo G, Smith A A genome-wide screen in EpiSCs identifies Nr5a nuclear receptors as potent inducers of ground state pluripotency. Development, 2010-10-01;137(19):3185-92. 2010-10-01 [PMID: 20823062] (Western Blot, Mouse) Western Blot
Mouse
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