Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in HeLa Cells.Immunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A at 10 u/mL, followed by goat ...read more
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Human Skeletal Muscle. Loading: 10 ul of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit ...read more
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Mouse Skeletal Muscle.Loading: 10 ul of lysates per lane.Antibodies: LMX1A, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit ...read more
Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Rat Skeletal Muscle.Loading: 10 u of lysates per lane.Antibodies: LMX1A, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG ...read more
Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in Mouse Brain Tissue.Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LMX1A antibody at 2 u/ml. Tissue ...read more
Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in Rat Skeletal Muscle Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody at 5 u/ml. ...read more
Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in Human Brain Meningioma.Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody at 2 u/ml. ...read more
Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A. The immunogen is located within amino acids 300 - 350 of LMX1A. Amino Acid Squence: CPQMPGDHMHPYGAEPL
Specificity
At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B.
Predicted Species
Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMX1A
Purity
Peptide affinity purified
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42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-41193 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for LMX1A Antibody - BSA Free
LIM homeobox transcription factor 1, alpha
LMX1.1
Background
Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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