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Lipin 1 Antibody - BSA Free

Images

 
Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.
Immunohistochemistry: LPIN1 Antibody [NB110-57150] - Staining of LPIN1 in mouse pancreas.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.8 mg/ml

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Lipin 1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide to an internal region (within residues 300-400) of the human LPIN1 protein. [Swiss-Prot# Q14693]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 1.0-2.0 ug/ml
Application Notes
This LPIN1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot analysis, where a and is seen at ~97 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB110-57150 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lipin 1 Antibody - BSA Free

  • DKFZp781P1796
  • EC 3.1.3.18
  • EC 3.1.3.4
  • EC 5.4.2.7
  • KIAA0188phosphatidate phosphatase LPIN1
  • Lipin 1
  • lipin-1
  • LPIN1
  • PAP1

Background

LPIN1 (phosphatidate phosphatase LPIN1) is expressed most abundantly in adipocytes and skeletal muscles wherein it play two major roles (i) in the cytoplasm, LPIN1 acts as a phosphatidate phosphatase 1 (PAP1) enzyme converting phosphatidic acid to diacylglycerol which is the precursor of both triglycerides and phospholipids (ii) in the nucleus, LPIN1 functions as a transcriptional co-activator through interaction with transcription factors which regulate the expression of genes involved in energy pathways. It can interact with MEF2C and LPIN1 interction with PPARA as well as PPARGC1A leads to a complex formation that modulates gene transcription. Three LPIN-1 protein isoforms are generated by alternative splicing, specifically involved in either PAP1 activity, transcriptional function or lipid droplets biogenesis. Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipin 1 Antibody (NB110-57150)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
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(2)
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Product General Protocols

View specific protocols for Lipin 1 Antibody (NB110-57150): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lipin 1 Antibody (NB110-57150). (Showing 1 - 1 of 1 FAQs).

  1. I want to use Lpin1 antibody for mouse liver paraffin sections (tumor also). What is your opinion about success of this staining? What is the suitable dilution for IHC?
    • NB110-57150 has been tested and validated for use in human, mouse and rat. It is therefore guaranteed to work in those species and should be a good antibody to use in your mouse sections. The recommended dilution for IHC on paraffin embedded sections is 1:100 to 1:250.
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Bioinformatics

Gene Symbol LPIN1