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LIMS2 Antibody

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Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP1-52185] - 3.75ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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LIMS2 Antibody Summary

Immunogen
Peptide with sequence C-RWSNMSDALA corresponding to internal region according to NP_001129509.2, NP_001154875.1.
Epitope
C-RWSNMSDALA
Specificity
This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIMS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.5 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot detection limit 1:8000
Application Notes
Western blot: Preliminary experiments gave an approx 80kDa band in Human Heart and Mouse Heart lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_001129509.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Spleen.
Publications
Read Publication using NBP1-52185.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LIMS2 Antibody

  • FLJ10044
  • ILK-binding protein
  • LIM and senescent cell antigen-like domains 2
  • LIM and senescent cell antigen-like-containing domain protein 2
  • LIM-like protein 2
  • Particularly interesting new Cys-His protein 2
  • PINCH2
  • PINCH-2

Background

LIMS2 is a focal adhesion protein that associates with integrin-linked kinase (ILK; MIM 602366), a multidomain protein that mediates multiple protein-protein interactions at adhesion sites between cells and the extracellular matrix (ECM).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIMS2 Antibody (NBP1-52185)(1)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIMS2
Entrez
Uniprot