Home » Lgr5/GPR49 » Lgr5/GPR49 Antibodies » Lgr5/GPR49 Antibody (CL13567)

Lgr5/GPR49 Antibody (CL13567)

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Staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Western blot analysis in human cell line SH-SY5Y.
Staining of human ovarian cancer (dysgerminoma) shows strong membranous and cytoplasmic positivity in tumor cells.
Staining of human cerebral cortex shows moderate membranous positivity in a subset of neurons.
Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong cytoplasmic positivity in tumor cells.
Orthogonal Strategies: Analysis in human fallopian tube and tonsil tissues using NBP3-24570 antibody. Corresponding LGR5 RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
CL13567
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

Order Details

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Lgr5/GPR49 Antibody (CL13567) Summary

Immunogen
This antibody was designed to target the recombinant protein Lgr5/GPR49, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (91%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LGR5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Reactivity Notes

Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Lgr5/GPR49 Antibody (CL13567)

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lgr5/GPR49
Antibody (CL13567)
NBP3-24570
Lgr5/GPR49 Antibody (CL13567)
Species: Hu, Mu
Applications: WB, IHC

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Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
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Bioinformatics

Gene Symbol LGR5