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Lgr5/GPR49 Antibody (889901) [Allophycocyanin]

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HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevent transfectants and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 APC-conjugated Monoclonal Antibody (Catalog # FAB82401A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevent transfectants and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 APC-conjugated Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
889901
Clonality
Monoclonal
Host
Rat
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB82401U-100UG
Alexa Fluor 405
FAB82401V-100UG
Alexa Fluor 488
FAB82401G-100UG
Alexa Fluor 532
FAB82401X
Alexa Fluor 594
FAB82401T-100UG
Alexa Fluor 647
FAB82401R-100UG
Alexa Fluor 700
FAB82401N-100UG
Alexa Fluor 750
FAB82401S-100UG
Allophycocyanin
FAB82401A-025UG
Allophycocyanin/Cy7
FAB82401APCCY7
Biotin
FAB82401B
CoraFluor 1
FAB82401CL1
DyLight 350
FAB82401D
DyLight 405
FAB82401E
DyLight 488
FAB82401K
DyLight 550
FAB82401L
DyLight 594
FAB82401M
DyLight 650
FAB82401W
DyLight 680
FAB82401Y
DyLight 755
FAB82401Z
FITC
FAB82401F
HRP
FAB82401H
Janelia Fluor 525
FAB82401JF525
Janelia Fluor 549
FAB82401I
Janelia Fluor 646
FAB82401J
Janelia Fluor 669
FAB82401JF669
PE/Atto594
FAB82401PEATT594
PE/Cy5.5
FAB82401PECY55
PE/Cy7
FAB82401PECY7
PerCP
FAB82401C
Phycoerythrin
FAB82401P-100UG
Phycoerythrin
FAB82401P-025UG
Unconjugated
MAB82401
mFluor Violet 450 SE
FAB82401MFV450
mFluor Violet 500 SE
FAB82401MFV500
mFluor Violet 610 SE
FAB82401MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB82401
Carrier Free
MAB82401
Carrier Free
MAB82401-SP
Carrier Free
FAB82401P-025UG
Carrier Free
FAB82401P-100UG
Carrier Free
FAB82401U-100UG
Carrier Free
FAB82401T-100UG
Carrier Free
FAB82401S-100UG
Carrier Free
FAB82401R-100UG
Carrier Free
FAB82401N-100UG
Carrier Free
FAB82401G-100UG
Carrier Free
FAB82401V-100UG
Carrier Free
FAB82401A-025UG
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Lgr5/GPR49 Antibody (889901) [Allophycocyanin] Summary

Immunogen
Mouse Lgr5/GPR49 N-terminal peptide
Ala21-Cys34
Accession # Q9Z1P4
Specificity
Detects mouse Lgr5/GPR49 in direct ELISAs. Stains mouse Lgr5/GPR49 transfected cells but not irrelevant transfectants in Flow Cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
LGR5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publications using
FAB82401A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lgr5/GPR49 Antibody (889901) [Allophycocyanin]

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine‑rich repeat G‑protein‑coupled Receptor 5 (Lgr5), also known as GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling. Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells. Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts. It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lgr5/GPR49
Antibody (889901) [Al ...
FAB82401A
Lgr5/GPR49 Antibody (889901) [Allophycocyanin]
Species: Mu
Applications: Flow

Publications for Lgr5/GPR49 Antibody (FAB82401A)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(2)
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(2)
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Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
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Bioinformatics

Gene Symbol LGR5
Uniprot