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Latexin Antibody

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Genetic Strategies: Western Blot: Latexin Antibody [NBP1-32525] - Wild-type (WT) and LXN knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody ...read more
Immunocytochemistry/ Immunofluorescence: LXN Antibody [NBP1-32525] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: LXN Antibody [NBP1-32525] - Paraffin-embedded FaDu xenograft, using antibody at 1:500 dilution.
Western Blot: LXN Antibody [NBP1-32525] - (30 ug whole cell lysate) A: 293T B: HeLa S3 12% SDS PAGE; antibody diluted at 1:1000
Western Blot: LXN Antibody [NBP1-32525] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Latexin Antibody [NBP1-32525] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Latexin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was ...read more
Western Blot: Latexin Antibody [NBP1-32525] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Latexin antibody [N1C3] (NBP1-32525) diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Latexin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Latexin. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LXN
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Latexin Antibody

  • ECI
  • ECIMUM
  • Endogenous carboxypeptidase inhibitor
  • Latexin
  • LXN
  • TCI
  • TCIProtein MUM
  • Tissue carboxypeptidase inhibitor

Background

Latexin is a specific inhibitor of zinc-dependent metallocarboxypeptidases (Pallares et al., 2005 [PubMed 15738388]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Latexin Antibody (NBP1-32525) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Latexin Products

Research Areas for Latexin Antibody (NBP1-32525)

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Blogs on Latexin.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

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Bioinformatics

Gene Symbol LXN