![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34663-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.")
| Reactivity | Hu, Pm, Ch(-), Mu(-), Rt(-)Species Glossary |
| Applications | Flow, ICC/IF |
| Clone | KU729 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34247). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Nuclear extract of human HL-60 cells |
| Localization | Nuclear |
| Marker | Nuclear Marker |
| Specificity | Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | XRCC6 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 75 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | XRCC6 |
![Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 format of this antibody.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Azide-and-BSA-Free-Flow-Intracellular-NBP2-34663-img0005.jpg "Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 format of this antibody.")
![Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Azide-and-BSA-Free-Flow-Intracellular-NBP2-34663-img0001.jpg "Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34663-img0003.jpg "Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).")
![Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-KU729-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34663-img0004.jpg "Flow Cytometry: Ku70/XRCC6 Antibody (KU729) - Azide and BSA Free [NBP2-34663] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")