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Kir3.1 Antibody (OTI1G3)

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Western Blot: Kir3.1 Antibody (1G3) [NBP2-01702] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kir3.1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kir3.1.
Western Blot: Kir3.1 Antibody (1G3) [NBP2-01702] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kir3.1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: Kir3.1 Antibody (1G3) [NBP2-01702] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Kir3.1.
Immunohistochemistry-Paraffin: Kir3.1 Antibody (1G3) [NBP2-01702] - Staining of paraffin-embedded Human prostate tissue using anti-Kir3.1 mouse monoclonal antibody.
Flow Cytometry: Kir3.1 Antibody (1G3) [NBP2-01702] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Kir3.1 antibody, and then ...read more
Immunohistochemistry-Paraffin: Kir3.1 Antibody (1G3) [NBP2-01702] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Kir3.1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Kir3.1 Antibody (1G3) [NBP2-01702] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Kir3.1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Kir3.1 Antibody (1G3) [NBP2-01702] - Staining of paraffin-embedded Human endometrium tissue using anti-Kir3.1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Kir3.1 Antibody (1G3) [NBP2-01702] - Staining of paraffin-embedded Human Kidney tissue using anti-Kir3.1 mouse monoclonal antibody.
Flow Cytometry: Kir3.1 Antibody (1G3) [NBP2-01702] - Analysis of Jurkat cells, using anti-Kir3.1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.51 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71065AF350
Alexa Fluor 405
NBP2-71065AF405
Alexa Fluor 488
NBP2-71065AF488
Alexa Fluor 532
NBP2-71065AF532
Alexa Fluor 594
NBP2-71065AF594
Alexa Fluor 647
NBP2-71065AF647
Alexa Fluor 700
NBP2-71065AF700
Alexa Fluor 750
NBP2-71065AF750
Allophycocyanin
NBP2-71065APC
Biotin
NBP2-71065B
CoraFluor 1
NBP2-71065CL1
DyLight 350
NBP2-71065UV
DyLight 405
NBP2-71065V
DyLight 488
NBP2-71065G
DyLight 550
NBP2-71065R
DyLight 594
NBP2-71065DL594
DyLight 650
NBP2-71065C
DyLight 680
NBP2-71065FR
DyLight 755
NBP2-71065IR
FITC
NBP2-71065F
HRP
NBP2-71065H
Janelia Fluor 525
NBP2-71065JF525
Janelia Fluor 549
NBP2-71065JF549
Janelia Fluor 585
NBP2-71065JF585
Janelia Fluor 635
NBP2-71065JF635
Janelia Fluor 646
NBP2-71065JF646
Janelia Fluor 669
NBP2-71065JF669
PE
NBP2-71065PE
PerCP
NBP2-71065PCP
mFluor Violet 450 SE
NBP2-71065MFV450
mFluor Violet 500 SE
NBP2-71065MFV500
mFluor Violet 610 SE
NBP2-71065MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71065
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Kir3.1 Antibody (OTI1G3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3(NP_002230) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KCNJ3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.51 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Kir3.1 Antibody (OTI1G3)

  • G protein-activated inward rectifier potassium channel 1
  • GIRK1
  • GIRK-1
  • GIRK1Kir3.1
  • Inward rectifier K(+) channel Kir3.1
  • inward rectifier K+ channel KIR3.1
  • KCNJ3
  • KGA
  • Kir3.1
  • Potassium channel, inwardly rectifying subfamily J member 3
  • potassium inwardly-rectifying channel, subfamily J, member 3

Background

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KCNJ3
Entrez