KIF2C Antibody (OTI1C5) - Azide and BSA Free

Images

 
Western Blot: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIF2C .
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Human Kidney tissue using anti-KIF2C mouse monoclonal antibody.
Western Blot: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KIF2C mouse monoclonal antibody.
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KIF2C mouse monoclonal antibody.
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KIF2C mouse monoclonal antibody.
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KIF2C mouse monoclonal antibody.
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIF2C mouse monoclonal antibody.
Immunohistochemistry: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - Staining of paraffin-embedded Human tonsil using anti-KIF2C mouse monoclonal antibody.
CyTOF-ready: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-KIF2C ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

KIF2C Antibody (OTI1C5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human KIF2C(NP_006836) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KIF2C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
81.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for KIF2C Antibody (OTI1C5) - Azide and BSA Free

  • kinesin family member 2C
  • kinesin-like 6 (mitotic centromere-associated kinesin)
  • Kinesin-like protein 6
  • kinesin-like protein KIF2C
  • MCAKKNSL6kinesin-like 6
  • Mitotic centromere-associated kinesin

Background

KIF2C is encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KIF2C