Keap1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP1-78793] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: Keap1 Antibody [NBP1-78793] - Staining of paraffin embedded Human Cerebellum with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Keap1 Antibody Summary

Immunogen
Peptide with sequence C-EVTPSQHGNRTFS corresponding to internal region (near N-Terminus) according to NP_036421.2.
Epitope
C-EVTPSQHGNRTFS
Specificity
Reported variants represent identical protein: NP_036421.2, NP_987096.1
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KEAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
Application Notes
IHC-P: Human cerebellum shows textured cytoplasm staining in Purkinje cell and processes.
Publications
Read Publication using NBP1-78793.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Keap1 Antibody

  • INrf2
  • INrf2MGC9454
  • Keap1
  • kelch-like ECH-associated protein 1
  • Kelch-like protein 19
  • KIAA0132MGC4407
  • KLHL19Cytosolic inhibitor of Nrf2
  • MGC10630
  • MGC1114
  • MGC20887

Background

The KEAP1 gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associatedprotein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins inthe cytoplasm is followed

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Keap1 Antibody (NBP1-78793)(1)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Keap1.


  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell, resulting in dysfunctional redox si...  Read full blog post.

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Bioinformatics

Gene Symbol KEAP1
Entrez
Uniprot