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Keap1 Antibody (OTI1B4)

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Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (OTI1B4) [NBP2-03319] - Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY KEAP1 using anti-KEAP1 antibody (green, upper left; DAPI/blue, lower ...read more
Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then ...read more
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: ...read more
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues used at a 1:200 dilution.
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of human BEAS2B cells.
Genetic Strategies: Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and KEAP1-Knockout Hela cells were separated by SDS-PAGE and ...read more
Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Hela cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Jurkat cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, KO
Clone
OTI1B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

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Keap1 Antibody (OTI1B4) Summary

Immunogen
Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KEAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot 1:500-2000
Theoretical MW
69.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-03319.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Keap1 Antibody (OTI1B4)

  • INrf2
  • INrf2MGC9454
  • Keap1
  • kelch-like ECH-associated protein 1
  • Kelch-like protein 19
  • KIAA0132MGC4407
  • KLHL19Cytosolic inhibitor of Nrf2
  • MGC10630
  • MGC1114
  • MGC20887

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Keap1 Antibody (NBP2-03319)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Keap1 Antibody (NBP2-03319) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Keap1.


  Read full blog post.

Crosstalk Between Oxidative Stress and Autophagy
By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell, resulting in dysfunctional redox si...  Read full blog post.

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Bioinformatics

Gene Symbol KEAP1
Entrez