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JNK2 Antibody [Unconjugated]

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Western blot shows lysates of C2C12 mouse myoblast cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). specific bands for JNK2 was detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of C2C12 mouse myoblast cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit ...read more
Western blot shows recombinant JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1846-SP
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JNK2 Antibody [Unconjugated] Summary

Immunogen
Human, Mouse, and Rat JNK2 synthetic peptide
PGIKFEELFPDWIFPSESERD
Specificity
Detects human, mouse, and rat JNK2 in Western blots. Recognizes p54 JNK (isoforms 1 and/or 4, both 424 aa) and p46 JNK (isoforms 2 and/or 3, both 382 aa) in Western blots. Because the peptide immunogen corresponds to a region of JNK2 with low homology to other JNKs, the antibody does not detect recombinant JNK1 or JNK3.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK2 Antibody [Unconjugated]

  • c-Jun kinase 2
  • c-Jun N-terminal kinase 2
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK2
  • JNK2A
  • JNK2BETA
  • JNK2JNK2ALPHA
  • JNK-55
  • Jun kinase
  • MAP kinase 9
  • MAPK 9
  • MAPK9
  • mitogen-activated protein kinase 9
  • p54a
  • p54aSAPK
  • PRKM9JNK2B
  • SAPK
  • SAPK1 alpha
  • Stress-activated protein kinase JNK2

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAPK9