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JAB1 Antibody (OTI2B12)

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Western Blot: JAB1 Antibody (2B12) [NBP2-01813] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY COPS5 (RC200979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiCOPS
Western Blot: JAB1 Antibody (2B12) [NBP2-01813] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY COPS5 (RC200979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: JAB1 Antibody (2B12) [NBP2-01813] - Anti-COPS5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COPS5(RC200979).
Immunohistochemistry-Paraffin: JAB1 Antibody (OTI2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Human bladder tissue within the normal limits using antiCOPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiCOPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Human Ovary tissue within the normal limits using antiCOPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric ...read more
Immunohistochemistry-Paraffin: JAB1 Antibody (OTI2B12) [NBP2-01813] - Staining of paraffin-embedded Human Kidney tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.76 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71659AF350
Alexa Fluor 405
NBP2-71659AF405
Alexa Fluor 488
NBP2-71659AF488
Alexa Fluor 532
NBP2-71659AF532
Alexa Fluor 594
NBP2-71659AF594
Alexa Fluor 647
NBP2-71659AF647
Alexa Fluor 700
NBP2-71659AF700
Alexa Fluor 750
NBP2-71659AF750
Allophycocyanin
NBP2-71659APC
Biotin
NBP2-71659B
CoraFluor 1
NBP2-71659CL1
DyLight 350
NBP2-71659UV
DyLight 405
NBP2-71659V
DyLight 488
NBP2-71659G
DyLight 550
NBP2-71659R
DyLight 594
NBP2-71659DL594
DyLight 650
NBP2-71659C
DyLight 680
NBP2-71659FR
DyLight 755
NBP2-71659IR
FITC
NBP2-71659F
HRP
NBP2-71659H
Janelia Fluor 525
NBP2-71659JF525
Janelia Fluor 549
NBP2-71659JF549
Janelia Fluor 585
NBP2-71659JF585
Janelia Fluor 635
NBP2-71659JF635
Janelia Fluor 646
NBP2-71659JF646
Janelia Fluor 669
NBP2-71659JF669
PE
NBP2-71659PE
PerCP
NBP2-71659PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71659
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JAB1 Antibody (OTI2B12) Summary

Immunogen
Full length human recombinant protein of human COPS5(NP_006828) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COPS5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.76 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JAB1 Antibody (OTI2B12)

  • COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
  • COP9 signalosome complex subunit 5
  • COPS5
  • CSN5
  • CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5
  • EC 3.4
  • JAB1
  • JAB138 kDa Mov34 homolog
  • Jun activation domain-binding protein 1
  • MOV-34
  • SGN5
  • SGN5MGC3149
  • Signalosome subunit 5

Background

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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JAB1 Antibody
(OTI2B12)
NBP2-01813
JAB1 Antibody (OTI2B12)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Bioinformatics

Gene Symbol COPS5
Entrez
Uniprot