![Knockout Validated: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Western-Blot-NBP2-16995-img0004.jpg "Knockout Validated: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ISG15/UCRP. The exact sequence is proprietary. |
| Localization | Cytoplasm; Secreted |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ISG15 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Western_Blot_5226.jpg)
![Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/16351.jpg)
![Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Flow_Cytometry_8189.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for ISG15/UCRP Antibody (NBP2-16995)Find related products by research area.
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| Gene Symbol | ISG15 |
![Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody [NBP2-16995] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min. Green: ISG15 protein stained by ISG15 antibody diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16995-img0006.jpg "Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody [NBP2-16995] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min. Green: ISG15 protein stained by ISG15 antibody diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: ISG15/UCRP Antibody [NBP2-16995] - Human gastric cancer, using ISG15 antibody antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Immunohistochemistry-Paraffin-NBP2-16995-img0002.jpg "Immunohistochemistry-Paraffin: ISG15/UCRP Antibody [NBP2-16995] - Human gastric cancer, using ISG15 antibody antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15/UCRP antibody (NBP2-16995) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16995_rabbit-polyclonal-isg15-ucrp-antibody-910202420122037.jpg "Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15/UCRP antibody (NBP2-16995) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")