IRE1 alpha Antibody

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha) (1). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator (2).

Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.

IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.

References

1. Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. (2019). ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. doi:10.1186/s12964-019-0353-3

2. Cho, Y. M., Kim, D. H., Lee, K. H., Jeong, S. W., & Kwon, O. J. (2018). The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102. doi:10.1038/s12276-018-0131-0

3. Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y., . . . Liu, J. (2019). Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 13811-13821. doi:10.1074/jbc.RA119.008353

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC-P, Knockdown Validated, WB, Western Blot.

Showing Publications 1 - 10 of 60. Show All 60 Publications. Collapse Publications.

Publications using NB100-2324	Applications	Species
Liao Y, Huang J, Wang Z et al. The phosphokinase activity of IRE1? prevents the oxidative stress injury through miR-25/Nox4 pathway after ICH CNS neuroscience & therapeutics 2023-11-23 [PMID: 37994671] (WB, Mouse)	WB	Mouse
Delmotte P, Yap J, Dasgupta D, Sieck G Chemical Chaperone 4-PBA Mitigates Tumor Necrosis Factor Alpha-Induced Endoplasmic Reticulum Stress in Human Airway Smooth Muscle International Journal of Molecular Sciences 2023-10-31 [PMID: 37958799] (WB, Human) Details: 1:1000 WB dilution	WB	Human
Wu D, Zhang X, Zimmerly KM et al. Unfolded protein response factor ATF6 augments T helper cell responses and promotes mixed granulocytic airway inflammation Mucosal Immunology 2023-08-01 [PMID: 37209959] (Western Blot)	Western Blot
Xiao J, Huang J, Jian X et al. IRE1? arm of unfolded protein response in muscle-specific TGF-? signaling-mediated regulation of muscle cell immunological properties Cellular & molecular biology letters 2023-02-27 [PMID: 36849929] (WB, Mouse)	WB	Mouse
Jin L, Pan M, Deng Y Et al. Effects of Shenling Baizhu powder on endoplasmic reticulum stress related signaling pathway in liver tissues of nonalcoholic fatty liver disease rats Traditional Medicine Research 2021-10-27
Moellmann J, Krueger K, Wong DWL et al. 2,8-Dihydroxyadenine-induced nephropathy causes hexosylceramide accumulation with increased mTOR signaling, reduced levels of protective SirT3 expression and impaired renal mitochondrial function Biochimica et biophysica acta. Molecular basis of disease 2023-08-01 [PMID: 37536502]
Wu D, Zhang X, Zimmerly KM et al. Unconventional Activation of IRE1 Enhances TH17 Responses and Promotes Neutrophilic Airway Inflammation bioRxiv : the preprint server for biology 2023-07-08 [PMID: 37461622] (WB, Mouse)	WB	Mouse
Espina M, Di Franco N, Brañas-Navarro M et al. The GRP78-PERK axis contributes to memory and synaptic impairments in Huntington's disease R6/1 mice Neurobiology of disease 2023-07-11 [PMID: 37442396] (WB, Mouse) Details: Dilution: 1:1000	WB	Mouse
Torrens JN, Hetzer SM, Evanson NK. Brief Oxygen Exposure after Traumatic Brain Injury Hastens Recovery and Promotes Adaptive Chronic Endoplasmic Reticulum Stress Responses International Journal of Molecular Sciences 2023-06-06 [PMID: 37372978]
Xie W, Jiang R, Xie J et al. Geometric Isomer of Guanabenz Confers Hepatoprotection to a Murine Model of Acetaminophen Toxicity Chemical research in toxicology 2023-06-22 [PMID: 37348131]
Wang X, Xin H, Zhang C et al. GRP94 Inhabits the Immortalized Porcine Hepatic Stellate Cells Apoptosis under Endoplasmic Reticulum Stress through Modulating the Expression of IGF-1 and Ubiquitin International Journal of Molecular Sciences 2022-11-14 [PMID: 36430538] (Western Blot)	Western Blot
M Oro?, M Grochowski, A Jaiswar, J Legierska, K Jastrz?bsk, M Nowak-Niez, M Ko?os, W Ka?miercza, T Olesi?ski, M Lenarcik, M Cybulska, M Mikula, A ?ylicz, M Mi?czy?ska, K Zettl, JR Wi?niewski, D Walerych The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells Cell Reports, 2022-09-27;40(13):111428. 2022-09-27 [PMID: 36170818]
Chakraborty S, Basu A miR-451a Regulates Neuronal Apoptosis by Modulating 14-3-3 zeta-JNK Axis upon Flaviviral Infection mSphere 2022-06-21 [PMID: 35727063] (WB, Mouse)	WB	Mouse
Wells KM, He K, Pandey A et al. Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense eLife 2022-05-19 [PMID: 35587649]
Li Q, Fan X, Lu W et al. Novel NPR2 Gene Mutations Affect Chondrocytes Function via ER Stress in Short Stature Cells 2022-04-08 [PMID: 35455946] (WB)	WB
Komatsu A, Iida I, Nasu Y et al. Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum Journal of Biological Chemistry 2022-05-01 [PMID: 35427648] (Western Blot)	Western Blot
Da L, Jeong P, Yu L et al. PERK prevents hepatic lipotoxicity by activating the p62-ULK1 axis-mediated noncanonical KEAP1-Nrf2 pathway. Redox Biol. 2022-01-14 [PMID: 35091323]
Srinivasan MP, Bhopale KK, Caracheo AA et al. Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase deficient deer mice American journal of physiology. Gastrointestinal and liver physiology 2022-01-05 [PMID: 34984929] (WB, Mouse)	WB	Mouse
Huang L, Liu Q, Zhou T Et al. Deficiency of beta -arrestin2 alleviates apoptosis through GRP78-ATF6-CHOP signaling pathway in primary Sjogren's syndrome International immunopharmacology 2021-10-29 [PMID: 34710848] (WB, Human)	WB	Human
Einstein Jm, Perelis M, Chaim Ia Et Al. Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer Molecular cell 2021-06-29 [PMID: 34216543] (WB)	WB
Qu Z, Lu X, Qu Y et al. Attenuation of the upregulation of NF‑kappa B and AP‑1 DNA‑binding activities induced by tunicamycin or hypoxia/reoxygenation in neonatal rat cardiomyocytes by SERCA2a overexpression International Journal of Molecular Medicine 2021-04-22 [PMID: 33907834] (WB, Rat)	WB	Rat
Pandey R, Bakay M, Strenkowski BP et al. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific reports 2021-04-01 [PMID: 33795715] (WB, Mouse)	WB	Mouse
Ding R, Ou W, Chen C et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation Neurochem. Int. 2020-09-24 [PMID: 32980492] (WB, Rat)	WB	Rat
Srinivasan MP, Bhopale KK, Caracheo AA et al. Pur Alpha regulates the induction of Znf179 transcription during neuronal differentiation Biochem Pharmacol 2020-10-01 [PMID: 32717227] (WB, Human, Rat)	WB	Human, Rat
Cicalese S, Okuno K, Elliott KJ et al. 78 kDa Glucose-Regulated Protein Attenuates Protein Aggregation and Monocyte Adhesion Induced by Angiotensin II in Vascular Cells Int J Mol Sci 2020-07-15 [PMID: 32679678] (WB, Rat)	WB	Rat
Bae SJ, Kim JE, Choi HJ et al. ?-Linolenic Acid-Enriched Cold-Pressed Perilla Oil Suppress High-Fat Diet-Induced Hepatic Steatosis through Amelioration of the ER Stress-Mediated Autophagy Molecules 2020-06-08 [PMID: 32521713]
Garufi A, Federici G, Gilardini Montani Ms et Al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation Biomolecules 2020-03-03 [PMID: 32138264] (WB, Human)	WB	Human
Han L, Bittner S, Dong D et al. Molecular changes in hepatic metabolism in ZDSD rats-A new polygenic rodent model of obesity, metabolic syndrome, and diabetes Biochim Biophys Acta Mol Basis Dis 2020-01-24 [PMID: 31987840] (WB, Rat)	WB	Rat
Schmit K, Chen JW, Ayama-Canden S et al. Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin Cell Death Dis 2019-12-04 [PMID: 31801939] (WB, Human)	WB	Human
Kim SM, Han Y, Yu SM, Kim SJ. Gallotannin attenuates 2?deoxy?D?glucose?induced dedifferentiation and endoplasmic reticulum stress through inhibition of inositol?requiring enzyme 1 downstream p38 kinase pathway in chondrocytes Molecular Medicine Reports 2019-10-25 [PMID: 31661132]
Gu R, Huang T, Xiao J et al. The IRE1 alpha Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation Front Physiol 2019-09-19 [PMID: 31607947] (WB, Mouse)	WB	Mouse
Srinivasan MP, Bhopale KK, Amer SM et al. Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice Biomolecules 2019-10-02 [PMID: 31581705] (WB, Mouse)	WB	Mouse
Xie W, Xie J, Vince R, More SS Guanabenz Attenuates Acetaminophen-Induced Liver Toxicity and Synergizes Analgesia in Mice Chem. Res. Toxicol. 2019-09-30 [PMID: 31524377]
Park SJ, Kim Y, Yang SM et al. Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome Proc. Natl. Acad. Sci. U.S.A. Jun 24 2019 [PMID: 31235574] (WB, Mouse)	WB	Mouse
Kaphalia L, Srinivasan MP, Kakumanu RD et al. Ethanol exposure impairs AMPK signaling and phagocytosis in human alveolar macrophages: Role of ethanol metabolism Alcohol. Clin. Exp. Res. 2019-06-18 [PMID: 31211863]
Inoue T, Yamakage H, Tanaka M et al. Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia Cells. 2019-05-31 [PMID: 31159306] (WB, Knockdown Validated, Mouse)	WB, Knockdown Validated	Mouse
Serrano, RL;Yu, W;Graham, RM;Bryan, RL;Terkeltaub, R; A vascular smooth muscle cell X-box binding protein 1 and transglutaminase 2 regulatory circuit limits neointimal hyperplasia PLoS ONE 2019-04-03 [PMID: 30943188] (IHC-P, Mouse)	IHC-P	Mouse
Hsu YH, Chuang HC, Lee YH et al. Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo Free Radic. Biol. Med. 2019-03-14 [PMID: 30878646] (IHC-P, Rat)	IHC-P	Rat
Lee MR, Bae SJ, Kim JE et al. Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris. Lab Anim Res 2018-12-01 [PMID: 30671117] (WB, Mouse)	WB	Mouse
Brenton-Romero R, Weisbrod R, Feng B et all. Liraglutide Treatment Reduces Endothelial Endoplasmic Reticulum Stress and Insulin Resistance in Patients With Diabetes Mellitus. J Am Heart Assoc 2018-09-18 [PMID: 30371206] (ICC/IF, Human)	ICC/IF	Human
Cho YM, Kim DH, Lee KH et al. The IRE1a-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPAR gamma activity Exp. Mol. Med. 2018-08-15 [PMID: 30111834] (WB, Mouse)	WB	Mouse
Yang F, Wang S, Liu Y et al. IRE1a aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells. Free Radic. Biol. Med. 2018-06-30 [PMID: 29969718] (WB, Mouse)	WB	Mouse
Tripathi M, Zhang CW, Singh BK et al. Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation. Cell Death Dis. 2016-12-08 [PMID: 27929536] (WB, Mouse)	WB	Mouse
Li Y, Zalzala M, Jadhav K et al. Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, ER stress and lipogenesis and is regulated by HNF4alpha. Hepatology. 2016-01-25 [PMID: 26806650]
Kim OK, Jun W, Lee J. Effect of Cudrania tricuspidata and Kaempferol in Endoplasmic Reticulum Stress-Induced Inflammation and Hepatic Insulin Resistance in HepG2 Cells. Nutrients. 2016-01-25 [PMID: 26805878] (WB, Human)	WB	Human
Vannuvel K, Van Steenbrugge M, Demazy C et al. Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population. J. Cell. Physiol. 2015-12-17 [PMID: 26680008] (WB, Human)	WB	Human
Alnasser HA, Guan Q, Zhang F et al. Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells. Am. J. Physiol. Renal Physiol. 2015-11-11 [PMID: 26561650] (WB, Mouse)	WB	Mouse
Quan X, Wang J, Liang C et al. Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells Biochem. Biophys. Res. Commun. 2015-06-12 [PMID: 26079879] (WB, Mouse) Details: IRE1 alpha antibody was used for WB on the lysates of C2C12 cells ( a primary line of murine myoblasts) that were serum-starved and treated with 0.5 ug/mL tunicamycin or without in the presence or absence of 100 nM melatonin or 10 uM Luzindole for 16 hours (Fig. 3).	WB	Mouse
Clarke JR, Lyra E Silva NM, Figueiredo CP et al. Alzheimer-associated Abeta oligomers impact the central nervous system to induce peripheral metabolic deregulation. EMBO Mol Med 2015-02-01 [PMID: 25617315] (WB)	WB
Ghosh Ak, Garg Sk, Mau T et al. Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging. J. Gerontol. A Biol. Sci. Med. Sci. 2014-10-16 [PMID: 25324219] (WB, Mouse)	WB	Mouse
Wu Y, Yang M, Fan J et al. Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis. Cell Death Dis. 2014-10-17 [PMID: 25321471] (WB, Mouse)	WB	Mouse
Liu L, Fan Z, Tang Y, Ke Z. The Resveratrol Attenuates Ethanol-Induced Hepatocyte Apoptosis Via Inhibiting ER-Related Caspase-12 Activation and PDE Activity In Vitro. Alcohol Clin Exp Res. 2013-11-13 [PMID: 24224909] (WB, Human)	WB	Human
Zheng M, Zhang Q, Joe Y et al. Carbon Monoxide Releasing Molecules Reverse Leptin Resistance Induced by Endoplasmic Reticulum Stress. Am J Physiol Endocrinol Metab 2013-02-12 [PMID: 23403944] (WB, Human, Mouse)	WB	Human, Mouse
Arshad M, Ye Z, Gu X et al. RNF13, a RING Finger Protein, Mediates Endoplasmic Reticulum Stress-induced Apoptosis through the Inositol-requiring Enzyme (IRE1alpha)/c-Jun NH2-terminal Kinase Pathway. J Biol Chem 2013-03-22 [PMID: 23378536] (WB, Human)	WB	Human
Kurata M, Yamazaki Y, Kanno Y et al. Anti-apoptotic function of Xbp1 as an IL-3 signaling molecule in hematopoietic cells. Cell Death and Dis. 2011-02-10 [PMID: 21368889] (WB, Mouse)	WB	Mouse
Luo D, He Y, Zhang H et al. AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response. J Biol Chem;283(18):11905-12. 2008-05-02 [PMID: 18281285] (WB, Mouse)	WB	Mouse
Lipson, KL et al. Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IER1. Cell Metabolism 4: 245-254. 2006-01-01 [PMID: 16950141] (WB, Mouse)	WB	Mouse
Fonesca, SG et al. WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells. J Biol Chem280(47):39609-15. 2005-11-25 [PMID: 16195229]
Kubota, K et al. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem;280(24):23194-202. 2005-06-17 [PMID: 15849362]
Show All 60 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	ICC	Mouse	11/19/2018	View Summary Application Immunocytochemistry Sample Tested mouse vascular smooth muscle cell Species Mouse Lot D-1
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	03/07/2018	View Summary Application Western Blot Sample Tested Liver Species Mouse Lot c1

Array NB100-2324

• IRE1 alpha Antibodies
• IRE1 alpha Antibody Pack
• IRE1 alpha ELISA Kits
• IRE1 alpha Proteins