Sheep Inhibin beta A ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity ShSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
2.7 pg/mL

Order Details

Sheep Inhibin beta A ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
2.7 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
86 - 95%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
INHBA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Sheep Inhibin beta A ELISA Kit (Colorimetric)

  • Activin Beta A
  • Activin beta-A chain
  • beta A (activin A, activin AB alpha polypeptide)
  • EDF
  • erythroid differentiation factor
  • Erythroid differentiation protein
  • follicle-stimulating hormone-releasing protein
  • FRP
  • FSH-releasing protein
  • INHBA
  • inhibin beta A chain
  • Inhibin Beta A
  • Inhibin Subunit Beta A
  • inhibin, beta A
  • Inhibin, beta-1

Background

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol INHBA