![Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Importin-alpha-3-KPNA4-Antibody-Western-Blot-NBP1-31260-img0019.jpg "Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE diluted at 1:1000")
| Reactivity | Hu, Mu, Rt, Bv, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin alpha 3/KPNA4. The exact sequence is proprietary. |
| Localization | Cytoplasm, Nucleus |
| Predicted Species | Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KPNA4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol (pH7) |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Verified Customer |
WB | Human | 08/31/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Analysis of HeLa, using KPNA4 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Importin-alpha-3-KPNA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31260-img0012.jpg "Immunocytochemistry/Immunofluorescence: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Analysis of HeLa, using KPNA4 antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - H1299 xenograft, using KPNA4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-alpha-3-KPNA4-Antibody-Immunohistochemistry-Paraffin-NBP1-31260-img0013.jpg "Immunohistochemistry-Paraffin: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - H1299 xenograft, using KPNA4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 7.5% SDS PAGE diluted at 1:150](http://images.novusbio.com/fullsize/Importin-alpha-3-KPNA4-Antibody-Western-Blot-NBP1-31260-img0015.jpg "Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 7.5% SDS PAGE diluted at 1:150")
![Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - A. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000](http://images.novusbio.com/fullsize/Importin-alpha-3-KPNA4-Antibody-Western-Blot-NBP1-31260-img0016.jpg "Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - A. 30 ug Rat2 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000")
![Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000](http://images.novusbio.com/fullsize/Importin-alpha-3-KPNA4-Antibody-Western-Blot-NBP1-31260-img0018.jpg "Western Blot: Importin alpha 3/KPNA4 Antibody [NBP1-31260] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
