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Canine IL-5 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
15.6 - 1000 pg/mL
Sensitivity
6.1 pg/mL

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Canine IL-5 ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
15.6 - 1000 pg/mL
Sensitivity
6.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
83 - 96%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Canine IL-5 ELISA Kit (Colorimetric)

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Canine IL-5 ELISA
Kit (Colorimetric)
NBP3-42558
Canine IL-5 ELISA Kit (Colorimetric)
Species: Ca
Applications: ELISA

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Bioinformatics

Gene Symbol IL5