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IL-5 Antibody (TRFK5) [Unconjugated]

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Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) followed by ...read more
Recombinant Mouse IL‑5 (Catalog # 405-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑5 (0.5 ng/mL) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications B/N, CyTOF-ready, ELISA(Cap), Flow
Clone
TRFK5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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IL-5 Antibody (TRFK5) [Unconjugated] Summary

Immunogen
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Specificity
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
IL5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.004-0.015 ug/mL
Application Notes
ELISA Detection: Mouse IL-5 Biotinylated Antibody (Catalog number BAM705)
Standard: Recombinant Mouse IL-5 (Catalog number 405-ML)
Publications
Read Publications using
MAB405 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-5 Antibody (TRFK5) [Unconjugated]

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB405
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Applications: B/N, CyTOF-ready, ELISA(Cap), Flow

Publications for IL-5 Antibody (MAB405)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Guinea Pig.

We have publications tested in 4 applications: ELISA Development, Immunoassay, Luminex Development, Neutralization.


Filter By Application
ELISA Development
(7)
Immunoassay
(1)
Luminex Development
(2)
Neutralization
(4)
All Applications
Filter By Species
Human
(4)
Mouse
(9)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB405 Applications Species
Yumiko Tanaka, Mai Yamagishi, Yasutaka Motomura, Takashi Kamatani, Yusuke Oguchi, Nobutake Suzuki et al. Time-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation Commun Biol 2023-09-06 [PMID: 37673922]
Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K; Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s Cell reports 2023-06-07 [PMID: 37294636] (Immunoassay, Human) Immunoassay Human
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Yamada T, Tani Y, Nakanishi H Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. FASEB J., 2010-10-19;25(2):561-8. 2010-10-19 [PMID: 20959515] (Neutralization, Mouse) Neutralization Mouse
Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5. Inflamm. Res., 2009-02-01;58(2):103-8. 2009-02-01 [PMID: 19184361] (Neutralization, Guinea Pig) Neutralization Guinea Pig
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-11-01;53(11):2010-2. 2007-11-01 [PMID: 18030696] (ELISA Development, Human) ELISA Development Human
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human) Luminex Development Human
Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients&#039; lungs through an allergen- and interleukin-5-dependent mechanism. Clin. Exp. Allergy, 2007-01-01;37(1):29-38. 2007-01-01 [PMID: 17210039] (ELISA Development, Mouse) ELISA Development Mouse
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (ELISA Development, Human) ELISA Development Human
Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006-06-01;168(6):1940-50. 2006-06-01 [PMID: 16723709] (ELISA Development, Mouse) ELISA Development Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol IL5