Recombinant Porcine IL-3 Protein Summary
Description |
Recombinant Pig Interleukin-3 (IL-3) is a recombinant protein corresponding to aa 24-144 of native pig IL-3. |
Preparation Method |
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography. |
Protein/Peptide Type |
Recombinant Protein |
Gene |
IL3 |
Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
13.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
10% Trehalose |
Concentration |
LYOPH |
Purity |
>95%, by SDS-PAGE |
Reconstitution Instructions |
Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. |
Alternate Names for Recombinant Porcine IL-3 Protein
Background
IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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