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IL-3 Antibody (OTI3B11) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3B11
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71023AF350
Alexa Fluor 405
NBP2-71023AF405
Alexa Fluor 488
NBP2-71023AF488
Alexa Fluor 532
NBP2-71023AF532
Alexa Fluor 594
NBP2-71023AF594
Alexa Fluor 647
NBP2-71023AF647
Alexa Fluor 700
NBP2-71023AF700
Alexa Fluor 750
NBP2-71023AF750
Allophycocyanin
NBP2-71023APC
Biotin
NBP2-71023B
DyLight 350
NBP2-71023UV
DyLight 405
NBP2-71023V
DyLight 488
NBP2-71023G
DyLight 550
NBP2-71023R
DyLight 594
NBP2-71023DL594
DyLight 650
NBP2-71023C
DyLight 680
NBP2-71023FR
DyLight 755
NBP2-71023IR
FITC
NBP2-71023F
HRP
NBP2-71023H
Janelia Fluor 525
NBP2-71023JF525
Janelia Fluor 549
NBP2-71023JF549
Janelia Fluor 585
NBP2-71023JF585
Janelia Fluor 635
NBP2-71023JF635
Janelia Fluor 646
NBP2-71023JF646
Janelia Fluor 669
NBP2-71023JF669
PE
NBP2-71023PE
PerCP
NBP2-71023PCP
Unconjugated
NBP1-47806
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47806
Azide and BSA Free
NBP2-71023
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IL-3 Antibody (OTI3B11) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IL-3 Antibody (OTI3B11) [CoraFluor™ 1]

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-3 Antibody
(OTI3B11) [CoraFluor& ...
NBP2-71023CL1
IL-3 Antibody (OTI3B11) [CoraFluor™ 1]
Species: Hu
Applications: WB, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL3