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IL-1ra/IL-1F3/IL1RN Antibody

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Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody diluted at 1:1000. ...read more
Immunocytochemistry/ Immunofluorescence: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Paraffin-embedded PC3 xenograft, using antibody at 1:500 dilution.
Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - A. 30 ug Neuro2A whole lysate/extract, B. 30 ug C8D30 whole cell lysate/extract, C. 30 ug NIH-3T3 whole cell lysate/extract. D. 30 ug Raw264. 7 whole cell ...read more
Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - A. 30 ug PC-12 whole cell lysate/extract15 % SDS-PAGE IL1 Receptor antagonist antibody dilution: 1:1000
Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - IL-1ra Antibody detects IL-1ra protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15 % SDS-PAGE, and blotted with IL-1ra ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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IL-1ra/IL-1F3/IL1RN Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IL-1ra/IL-1F3/IL1RN. The exact sequence is proprietary.
Localization
Isoform 1: Secreted, Isoform 2: Cytoplasm, Isoform 3: Cytoplasm, Isoform 4: Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1RN
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-32568.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (83%), Porcine (82%), Bovine (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IL-1ra/IL-1F3/IL1RN Antibody (NBP1-32568)(2)

We have publications tested in 1 confirmed species: Human.


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Reviews for IL-1ra/IL-1F3/IL1RN Antibody (NBP1-32568) (0)

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Product General Protocols

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Video Protocols

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FAQs for IL-1ra/IL-1F3/IL1RN Antibody (NBP1-32568) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL1RN