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Recombinant Mouse IL-19 Protein

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SDS-Page: Recombinant Mouse IL-19 Protein [NBP2-61330]
SDS-Page: Recombinant Mouse IL-19 Protein [NBP2-61330]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Bioactivity
Concentration
LYOPH

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Recombinant Mouse IL-19 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-19 Source: E. coli

Uniprot ID: Q8CJ70

Amino Acid Sequence: LRRCLISVDM RLIEKSFHEI KRAMQTKDTF KNVTILSLEN LRSIKPGDVC CMTNNLLTFY RDRVFQDHQE RSLEVLRRIS SIANSFLCVQ KSLERCQVHR QCNCSQEATN ATRIIHDNYN QLEVSSAALK SLGELNILLA WIDRNHLETP AA
Details of Functionality
IL-19 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Ra and human IL-20Rb co-transfected murine BaF3 pro-B cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL19
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of IL-19 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Western Blot
Theoretical MW
17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 3% Trehalose.
Preservative
Trehalose
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Mouse IL-19 Protein

  • IL-10C
  • IL19
  • IL-19
  • interleukin 19
  • MDA1
  • melanoma differentiation associated protein-like protein
  • NG.1Melanoma differentiation-associated protein-like protein
  • ZMDA1interleukin-19

Background

IL19 is encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL19