IL-12 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Affinity Chromatography, Bioassay, ICC, IHC, Neutralization.

Showing Publications 1 - 10 of 20. Show All 20 Publications. Collapse Publications.

Publications using AF-219-NA	Applications	Species
Jürgens, M;Claus, M;Wingert, S;Niemann, JA;Picard, LK;Hennes, E;Haasler, I;Hellwig, B;Overbeck, N;Reinders, J;Rahnenführer, J;Schedel, M;Capellino, S;Watzl, C; Chronic stimulation desensitizes ?2-adrenergic receptor responses in natural killer cells European journal of immunology 2024-09-30 [PMID: 39350450] (Neutralization, Human)	Neutralization	Human
Ming-Na Zhao, Ling-Fei Zhang, Zhen Sun, Li-Hua Qiao, Tao Yang, Yi-Zhe Ren et al. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer Cell Death & Disease 5/1/2023 [PMID: 37127752]
SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs Nature Immunology, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456734] (Neutralization, Human)	Neutralization	Human
Yost E, Hynes T, Hartle C, Ott B, Berlot C Inhibition of G-protein betagamma signaling enhances T cell receptor-stimulated interleukin 2 transcription in CD4+ T helper cells. PLoS ONE, 2015-01-28;10(1):e0116575. 2015-01-28 [PMID: 25629163] (Bioassay, Human)	Bioassay	Human
Rashmi Jalah, Margherita Rosati, Brunda Ganneru, Guy R. Pilkington, Antonio Valentin, Viraj Kulkarni et al. The p40 subunit of interleukin (IL)-12 promotes stabilization and export of the p35 subunit: implications for improved IL-12 cytokine production Journal of Biological Chemistry 3/1/2013 [PMID: 23297419]
Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells. Int. Immunol., 2008-08-13;20(10):1321-9. 2008-08-13 [PMID: 18703465] (Neutralization, Human)	Neutralization	Human
Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A Upregulated IL-23 and IL-17 in Behcet patients with active uveitis. Invest. Ophthalmol. Vis. Sci., 2008-07-01;49(7):3058-64. 2008-07-01 [PMID: 18579762] (Neutralization, Human)	Neutralization	Human
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008-04-01;38(4):1012-23. 2008-04-01 [PMID: 18350544] (Neutralization, Human)	Neutralization	Human
Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes. J. Immunol., 2006-05-01;176(9):5284-92. 2006-05-01 [PMID: 16621994] (Neutralization, Human)	Neutralization	Human
Koelle DM, Huang J, Hensel MT, McClurkan CL Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes. J. Virol., 2006-03-01;80(6):2863-72. 2006-03-01 [PMID: 16501095] (Neutralization, Human)	Neutralization	Human
Nagarajan S, Selvaraj P Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination. Vaccine, 2005-12-07;24(13):2264-74. 2005-12-07 [PMID: 16376465] (Affinity Chromatography, Human)	Affinity Chromatography	Human
Tomoki Ito, Yui-Hsi Wang, Omar Duramad, Toshiyuki Hori, Guy J. Delespesse, Norihiko Watanabe et al. TSLP-activated dendritic cells induce an inflammatory T helper type 2 cell response through OX40 ligand The Journal of Experimental Medicine 11/7/2005 [PMID: 16275760]
Takei M, Umeyama A, Arihara S, Matsumoto H Effect of piceatannol in human monocyte-derived dendritic cells in vitro. J Pharm Sci, 2005-05-01;94(5):974-82. 2005-05-01 [PMID: 15793803] (Neutralization, Human)	Neutralization	Human
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism. J. Immunol., 2005-02-15;174(4):2376-86. 2005-02-15 [PMID: 15699174] (Neutralization, Human)	Neutralization	Human
Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10. J. Immunol., 2004-05-01;172(9):5213-21. 2004-05-01 [PMID: 15100259] (Neutralization, Human)	Neutralization	Human
Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA. J. Immunol., 2004-01-01;172(1):138-43. 2004-01-01 [PMID: 14688319] (Neutralization, Human)	Neutralization	Human
Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen. J. Immunol., 2004-01-01;172(1):130-7. 2004-01-01 [PMID: 14688318] (Neutralization, Human)	Neutralization	Human
Artavanis-Tsakonas K, Eleme K, McQueen KL, Cheng NW, Parham P, Davis DM, Riley EM Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes. J. Immunol., 2003-11-15;171(10):5396-405. 2003-11-15 [PMID: 14607943] (Neutralization, Human)	Neutralization	Human
Hessian PA, Highton J, Kean A, Sun CK, Chin M Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Arthritis Rheum., 2003-02-01;48(2):334-8. 2003-02-01 [PMID: 12571841] (IHC, Human)	IHC	Human
Nutku E, Gounni AS, Olivenstein R, Hamid Q Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma. J. Allergy Clin. Immunol., 2000-08-01;106(2):288-92. 2000-08-01 [PMID: 10932072] (ICC, Human)	ICC	Human
Show All 20 Publications. Collapse Publications.

Array AF-219-NA

• IL-12 Antibodies
• IL-12 Antibody Pair
• IL-12 ELISA Kits
• IL-12 Proteins and Enzymes