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IL-11 Antibody (188520)

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Recombinant Mouse IL‑11 (Catalog # 418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑11 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clone
188520
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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IL-11 Antibody (188520) Summary

Immunogen
E. coli-derived recombinant mouse IL-11
Gly23-Leu199
Accession # P47873
Specificity
Detects mouse IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10‑20% cross-reactivity with recombinant human (rh) IL-11 is observed and no cross-reactivity with recombinant rat CNTF, recombinant mouse (rm) IL-6, rmLIF, rmOSM, rmCT-1, or rhCLC is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL11
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.5-2.0 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB418 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-11 Antibody (188520)

  • Adipogenesis inhibitory factor
  • AGIF
  • AGIFoprelvekin
  • IL11
  • IL-11
  • IL-11Oprelvekin
  • interleukin 11
  • interleukin-11
  • Oprelvekin

Background

Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The mouse IL-11 cDNA encodes a 199 amino acid precursor polypeptide with a 22 amino acid hydrophobic signal that is processed proteolytically to generate the 177 amino acid mature protein. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cells. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute‑phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of human IL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor alpha chain and gp130.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-11 Antibody (MAB418)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC, Neutralization.


Filter By Application
IHC
(1)
Neutralization
(3)
All Applications
Filter By Species
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using MAB418 Applications Species
X Zhang, N Kiapour, S Kapoor, T Khan, M Thamilaras, Y Tao, S Cohen, R Miller, RA Sobel, S Markovic-P IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis J. Immunol., 2019-07-24;0(0):. 2019-07-24 [PMID: 31341075] (Neutralization, Mouse) Neutralization Mouse
Susanta Mondal, Malabendu Jana, Sridevi Dasarathi, Avik Roy, Kalipada Pahan Aspirin ameliorates experimental autoimmune encephalomyelitis through interleukin-11–mediated protection of regulatory T cells Science Signaling 2018-11-29 [PMID: 30482850]
Ahrom Ham, Mihwa Kim, Joo Yun Kim, Kevin M. Brown, James Yeh, Vivette D. D’Agati et al. Critical Role of Interleukin-11 in Isoflurane-mediated Protection against Ischemic Acute Kidney Injury in Mice Anesthesiology 2013-12-01 [PMID: 24037316]
Anurag Maheshwari, Kris Janssens, Jeroen Bogie, Chris Van Den Haute, Tom Struys, Ivo Lambrichts et al. Local Overexpression of Interleukin-11 in the Central Nervous System Limits Demyelination and Enhances Remyelination Mediators of Inflammation 2013-05-30 [PMID: 23818742]
Kim J, Kim M, Ham A, Brown K, Greene R, D'Agati V, Lee H IL-11 is required for A1 adenosine receptor-mediated protection against ischemic AKI. J Am Soc Nephrol, 2013-06-27;24(10):1558-70. 2013-06-27 [PMID: 23813214] (IHC, Neutralization, Mouse) IHC, Neutralization Mouse
Mitchell HM, White DM, Domowicz MS, Kraig RP Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11. J. Neurochem., 2010-12-01;117(2):187-96. 2010-12-01 [PMID: 21070241] (Neutralization, Rat) Neutralization Rat

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Bioinformatics

Gene Symbol IL11
Uniprot