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Mouse IL-1 RI ELISA Kit (Colorimetric)

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ELISA: Mouse IL-1 RI ELISA Kit (Colorimetric) [NBP3-06803] - Samples were spiked with high concentrations of Mouse IL-1 RI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse IL-1 RI ELISA Kit (Colorimetric) [NBP3-06803] - Samples were spiked with high concentrations of Mouse IL-1 RI and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Mouse IL-1 RI ELISA Kit (Colorimetric) [NBP3-06803] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
93.75 - 6000 pg/mL
Sensitivity
56.25 pg/mL

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Mouse IL-1 RI ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse IL-1 RI in samples. No significant cross-reactivity or interference between Mouse IL-1 RI and analogues was observed.
Standard Curve Range
93.75 - 6000 pg/mL
Sensitivity
56.25 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.66
Intra-Assay
4.88
Spike Recovery
86 - 106 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL1R1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse IL-1 RI ELISA Kit (Colorimetric)

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  SDS
  Mouse IL-1 RI ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol IL1R1