Home » IL-1 alpha/IL-1F1 » IL-1 alpha/IL-1F1 Antibodies » IL-1 alpha/IL-1F1 Antibody (OTI2F8) [CoraFluor™ 1]
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IL-1 alpha/IL-1F1 Antibody (OTI2F8) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2F8
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71021AF350
Alexa Fluor 405
NBP2-71021AF405
Alexa Fluor 488
NBP2-71021AF488
Alexa Fluor 532
NBP2-71021AF532
Alexa Fluor 594
NBP2-71021AF594
Alexa Fluor 647
NBP2-71021AF647
Alexa Fluor 700
NBP2-71021AF700
Alexa Fluor 750
NBP2-71021AF750
Allophycocyanin
NBP2-71021APC
Biotin
NBP2-71021B
DyLight 350
NBP2-71021UV
DyLight 405
NBP2-71021V
DyLight 488
NBP2-71021G
DyLight 550
NBP2-71021R
DyLight 594
NBP2-71021DL594
DyLight 650
NBP2-71021C
DyLight 680
NBP2-71021FR
DyLight 755
NBP2-71021IR
FITC
NBP2-71021F
HRP
NBP2-71021H
Janelia Fluor 525
NBP2-71021JF525
Janelia Fluor 549
NBP2-71021JF549
Janelia Fluor 646
NBP2-71021JF646
Janelia Fluor 669
NBP2-71021JF669
PE
NBP2-71021PE
PerCP
NBP2-71021PCP
Unconjugated
NBP2-45400
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45400
Azide and BSA Free
NBP2-71021
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IL-1 alpha/IL-1F1 Antibody (OTI2F8) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL1A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IL-1 alpha/IL-1F1 Antibody (OTI2F8) [CoraFluor™ 1]

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-1 alpha/IL-1F1
Antibody (OTI2F8) [Co ...
NBP2-71021CL1
IL-1 alpha/IL-1F1 Antibody (OTI2F8) [CoraFluor™ 1]
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL1A