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Mouse IgG3 Isotype Control (MG3) [Rhodamine]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
MG3
Clonality
Monoclonal
Host
Mouse
Conjugate
Rhodamine
Concentration
LYOPH

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Mouse IgG3 Isotype Control (MG3) [Rhodamine] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Rhodamine Conjugated Mouse IgG3 was proven by Dot Blot.
Specificity
Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Rhodamine Conjugated Mouse IgG3 was proven by Dot Blot.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
IGHG3
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Flow cytometry, Immunofluorescence, FLISA, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent).

Alternate Names for Mouse IgG3 Isotype Control (MG3) [Rhodamine]

  • DKFZp686H11213
  • FLJ39988
  • FLJ40036
  • FLJ40253
  • FLJ40587
  • FLJ40789
  • FLJ40834
  • IgG3
  • immunoglobulin heavy constant gamma 3 (G3m marker)
  • MGC45809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol IGHG3