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Mouse IgG3 Isotype Control (MG3)

Images

 
Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Mouse IgG3. Load: 1ug per lane. ...read more
Dot Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose. Image from the FITC version ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, DB, ELISA, Flow, ICC/IF
Clone
MG3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Purified

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Mouse IgG3 Isotype Control (MG3) Summary

Description
This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Specificity
Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
IGHG3
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for Mouse IgG3 Isotype Control (MG3)

  • DKFZp686H11213
  • FLJ39988
  • FLJ40036
  • FLJ40253
  • FLJ40587
  • FLJ40789
  • FLJ40834
  • IgG3
  • immunoglobulin heavy constant gamma 3 (G3m marker)
  • MGC45809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG3 Isotype Control (NBP1-96777). (Showing 1 - 1 of 1 FAQ).

  1. Could you please let me know the supply form (Liquid or Lyohpilized) of this antibody? 
    • Yes, it is  liquid. Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Stabilizer: none. Preservative: 0.01% Sodium Azide 

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Bioinformatics

Gene Symbol IGHG3