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Recombinant Human IGFBP-rp1/IGFBP-7 Protein

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SDS-Page: Human IGFBP-rp1/IGFBP-7 Protein [NBP2-35007]
SDS-Page: Human IGFBP-rp1/IGFBP-7 Protein [NBP2-35007]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35007-1mg
Carrier-Free
NBP2-35007-100ug
Carrier-Free
NBP2-35007-500ug
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Recombinant Human IGFBP-rp1/IGFBP-7 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 256 amino acids corresponding to IGFBP-rp1/IGFBP-7 Source: E. coli

Uniprot ID: Q16270

Amino Acid Sequence: SSSDTCGPCE PASCPPLPPL GCLLGETRDA CGCCPMCARG EGEPCGGGGA GRGYCAPGME CVKSRKRRKG KAGAAAGGPG VSGVCVCKSR YPVCGSDGTT YPSGCQLRAA SQRAESRGEK AITQVSKGTC EQGPSIVTPP KDIWNVTGAQ VYLSCEVIGI PTPVLIWNKV KRGHYGVQRT ELLPGDRDNL AIQTRGGPEK HEVTGWVLVS PLSKEDAGEY ECHASNSQGQ ASASAKITVV DALHEIPVKK GEGAEL

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Preparation
Method
This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IGFBP7
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of IGFBP-rp1/IGFBP-7 as determined by LAL method.

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
26.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human IGFBP-rp1/IGFBP-7 Protein

  • angiomodulin
  • FSTL2
  • IBP-7
  • IGFBP7
  • IGFBP-7
  • IGFBP-7PGI2-stimulating factor
  • IGFBP-7v
  • IGFBPrp1
  • IGFBP-rp1
  • insulin-like growth factor binding protein 7
  • insulin-like growth factor-binding protein 7
  • MAC25 protein
  • Mac25
  • MAC25IGF-binding protein 7
  • Prostacyclin-stimulating factor
  • PSF
  • PSFAGM
  • TAF
  • Tumor-derived adhesion factor

Background

IGF-BP7, also named IBP-7, Mac25 and IGFBPrP1, is belonging to the superfamily of insulin-like growth factor (IGF) binding proteins and is encoded by the igfbp7 gene in human. It is expressed in a wide range of normal human tissues and it generally shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin. IGF-BP7 includes conserved cysteine residues. IGF-BP7 modulates the biological activities of IGF proteins. It also suppresses growth and colony formation of prostate and breast cancer cell lines. Above all, IGF-BP7 is a very important factor in skeletal myogenesis. Human IGF-BP7 cDNA encodes 282 amino acid (a.a.) residue precursor protein with a putative 26 a.a. signal peptide. Human and murine IGF-BP7 share 94% a.a. sequence identity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IGFBP7
Uniprot