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IFN-beta Antibody (1176D) [Unconjugated]

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Recombinant Mouse IFN‑ beta (Catalog # 8234-MB) reduces the viral-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Inhibition of viral activity elicited by Recombinant Mouse IFN‑ beta (10 pg/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IFN‑ beta Monoclonal Antibody (Catalog # MAB8234). The ND50 is typically0.25-1.25 µg/mL.
Recombinant Mouse IFN‑ beta (Catalog # 8234-MB) reduces the viral-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N
Clone
1176D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8234-SP
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IFN-beta Antibody (1176D) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human embryonic kidney cell line HEK293-derived mouse IFN‑ beta
Ile22-Asn182
Accession # P01575
Specificity
Detects mouse IFN‑ beta in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.25-1.25 ug/mL
Publications
Read Publication using
MAB8234 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-beta Antibody (1176D) [Unconjugated]

  • Fibroblast interferon
  • IFB
  • IFBIFNB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Teige, I. et al. (2003) J. Immunol. 170:4776.
  6. Shinohara, M.L. et al. (2008) Immunity 29:68.
  7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  11. Wang, H. et al. (2011) J. Immunol 186:675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IFN-beta Antibody
(1176D) [Unconjugated ...
MAB8234
IFN-beta Antibody (1176D) [Unconjugated]
Species: Mu
Applications: B/N

Publications for IFN-beta Antibody (MAB8234)(1)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 1 application: In vivo assay.


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FAQs for IFN-beta Antibody (MAB8234) (0)

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