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IFI35 Antibody (OTI1C9)

Images

 
Western Blot: IFI35 Antibody (1C9) [NBP2-01108] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFI35 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: IFI35 Antibody (1C9) [NBP2-01108] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFI35.
Immunohistochemistry-Paraffin: IFI35 Antibody (1C9) [NBP2-01108] - Staining of paraffin-embedded Human pancreas tissue using anti-IFI35 mouse monoclonal antibody.
Flow Cytometry: IFI35 Antibody (1C9) [NBP2-01108] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFI35 antibody, and then analyzed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.4 mg/ml

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IFI35 Antibody (OTI1C9) Summary

Immunogen
Full length human recombinant protein of human IFI35(NP_005524)produced in HEk293T cell
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IFI35
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IFI35 Antibody (OTI1C9)

  • FLJ21753
  • ifi-35
  • IFP 35
  • IFP35interferon-induced 35 kDa protein
  • interferon-induced protein 35

Background

IFI35, also known as Interferon-induced 35 kDa protein, consists of 2 isoforms, a 286 amino acid isoform 1 that is 31.5 kDa and a 188 amino acid isoform that is 32 kDa, has nucleus subcellular location and can be found in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Its function is not yet known. Studies of this protein are being performed on research about diverticulitis and ovarian cancer. This protein plays a role in interferon alpha/beta signaling, cytokine signaling in immune system, expression of IFN-induced genes, interferon signaling, and immune system pathways where it interacts with CLEC4G, BATF, PLEKHO1, NMI, and MAPK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IFI35
Entrez